乳酸杆菌对重型颅脑损伤大鼠胃肠动力障碍的影响

Influence of lactobacillus on gastrointestinal motility disturbance of rats with severe craniocerebral injury

[目的]探讨乳酸杆菌对重型颅脑损伤大鼠胃肠动力障碍的影响。[方法]建立重型颅脑损伤大鼠模型,将SD大鼠随机分为4组,肠内营养组给予三九全营素,乳酸杆菌组给予三九全营素加乳酸杆菌,合生元组给予三九全营素加乳酸杆菌、纤维素,假手术组给予正常饮食,于伤后第1天、第3天、第7天通过灌服葡聚糖兰-2000,检测大鼠胃排空、小肠推进功能,并动态观察大鼠的体重变化。[结果]肠内营养组于伤后第1天、第3天及乳酸杆菌组、合生元组于伤后第1天胃排空率和小肠传输率较假手术组显著下降(P<0.01或P<0.05),但伤后第3天,乳酸杆菌组的胃排空率和肠传输速率都已接近假手术组水平(P>0.05),而合生元组肠传输率仍低于假手术组(P<0.05);乳酸杆菌组、合生元组伤后第3天胃排空率和肠传输率速率较肠内营养组显著加快(P<0.05);伤后第7天,4组间的胃排空及肠传输速率差异均无统计学意义。致伤大鼠体重在1周内均持续下降。[结论]乳酸杆菌对重型颅脑伤大鼠的早期胃肠动力障碍有改善作用。

Objective：To probe into the influence of lactobacillus on gastro-intestinal motility disturbance of rats with severe craniocerebral injury.Methods：Severe craniocerebral injury rats model were prepared.And a total of 60 male SD rats were divided into four groups randomly,enteral nutrition group（3-9 entire nutrients）,lactobacillus group（3-9 entire nutrients adding to lactobacillus）,probiotics group（3-9 entire nutrients＋lactobacillus＋fiber）,and sham-operation group（normal diet）.Then gastric emptying and intestinal transmission rate of all rats who were filled with dextran blue-2000 on the first day,the third day,the seventh day after injury were detected respectively.The body weight of rats was also recorded dynamically.Results：Compared with sham-operation group,both the gastric emptying and the intestinal transmission rate were significantly reduced in rats on the first day after injury in lactobacillus group and probiotics group,on the third day after injury in enteral nutrition group（P0.05 or P0.01）.On the third day after injury,gastric emptying and intestinal transmission rate in lactobacillus group rats were closed to sham-operation group（P0.05）.However,the intestinal transmission rate in probiotics group was still different with sham-operation group on the third day after injury（P0.05）.Compared with enteral nutrition group,gastric emptying,intestinal transmission rate were significantly increased on the third day after injury in lactobacillus group and probiotics group（P0.05）.On the seventh day after injury,there was no statistical significant difference in terms of gastric emptying and intestinal transmission rate among all groups.The body weight of all rat models decreased persistently.However,there was no statistical significant difference among all groups（P0.05）.Conclusion：Lactobacillus can improve early gastrointestinal motility disturbance of rats with severe craniocerebral injury.