摘要
[目的]检测柚皮苷溶液对成骨细胞株MC3T3-E1细胞增殖功能的影响。[方法]以成骨细胞株MC3T3-E1细胞为体外药效的试验模型,通过CCK-8法观察各种浓度的柚皮苷溶液对MC3T3-E1细胞增殖能力的影响。[结果]高浓度的柚皮苷溶液在12h和24h时能促进MC3T3-E1细胞的增殖作用。而低浓度的柚皮苷溶液则无此作用。但是48h时各个浓度的柚皮苷溶液均不能促进细胞增殖。所测的各个组别细胞毒性百分比都较小,且随着时间的推移(12h,24h,48h)变化较小。光镜下观察各个时间点细胞的密度和形态也未发生明显的变化。[结论]柚皮苷溶液可以提高成骨细胞株MC3T3-E1增殖能力。
[Objective]To observe the effect of naringin solution on osteoblast line MC3T3-E1 proliferation.[Method]Take MC3T3-E1 as test model in vitro potency,observe the influence of all concentrations of naringin solutions on MC3T3-E1 proliferation with CCK-6 method.[Result]High concentration of narigin solution can promote MC3T3-E1 proliferation at 12h and 24h;while the low concentration of that has no such action.But at 48h,all concentrations of the solutions can not promote cell proliferation.The tested groups of cell toxin all have small percentage,and have little change with time passing by（12h,24,48h）.Under microscope,the cell concentration and shape have no marked change either.[Conclusion]Narigin solution can improve the osteoblast line MC3T3-E1 proliferation ability.
出处
《浙江中医药大学学报》
CAS
2010年第2期171-172,共2页
Journal of Zhejiang Chinese Medical University
关键词
柚皮苷
成骨细胞
骨碎补
增殖
narigin
osteoblast
Rhizoma Drynariae
proliferation
