苦瓜多糖抗肿瘤及免疫增强活性的研究

Study on the Antitumor and Immune-stimulating Activity of Polysaccharide from Momordica charantia

目的:研究苦瓜多糖的体内抗肿瘤及增强免疫作用,探讨可能作用机制。方法:通过建立小鼠S180肉瘤、H22肝癌肿瘤模型观察苦瓜多糖的抗肿瘤作用,此外应用MTT法观察苦瓜多糖对小鼠脾淋巴细胞增殖作用的影响,应用比色法观察苦瓜多糖对小鼠单核巨噬细胞RAW264.7吞噬功能的影响,运用Griess试剂检测苦瓜多糖促进小鼠单核巨噬细胞RAW264.7分泌NO含量的变化。结果:苦瓜多糖高中低剂量组能明显抑制小鼠S180肉瘤、H22肝癌肿瘤的生长,同时能明显增加荷瘤小鼠的脾腺指数和胸腺指数。此外苦瓜多糖能显著刺激小鼠脾淋巴细胞增殖,明显提高小鼠单核巨噬细胞RAW264.7吞噬中性红的能力,明显促进小鼠单核巨噬细胞RAW264.7分泌NO。结论:推测苦瓜多糖具有较强的免疫增强活性并可能通过刺激淋巴细胞、并增强巨噬细胞的活化来调节机体的免疫功能,从而抑制肿瘤细胞的生长。

Objective： To study the antitumor activity Momordica charantia （MCP） and its potential mechanism. and immunity enhancement of polysaccharide from Methods： The model of S180-bearing and H22- bearing tumor mice was built to study the antitumor activity of MCP in vivo. Besides, the stimulation effect of MCP on normal mice lymphocyte proliferation was assayed by MTr method, the phagocytic ability of macrophage RAW264.7 was evaluated with neutral red dye phagocytosis and the stimulation effect of MCP on NO production by macrophage RAW264.7 was measured by Griess reagents. Results： MCP could inhibit the growth of S180 and H22 tumor in vivo and increase the spleen gland index and thymus gland index of tumor-bearing mice. Furthermore, pharmacological experiments showed that MCP can promote normal mice lymphocyte proliferation and the ability of macrophage RAW264.7 in phagocytizing neutral red, and raising the level of NO production. Conclusion： The immune-stimulating effect, such as promoting lymphocyte proliferation and activating macrophage, may result in the antitumor activity of MCP.