摘要
目的:通过观察经前平颗粒对经前期综合征(PMS)肝气逆证大鼠下丘脑、顶区皮质5羟色胺和多巴胺受体亚型表达的影响,探讨经前平颗粒治疗PMS肝气逆证的机制。方法:制备PMS肝气逆症大鼠模型,经前平给药后心脏灌流4%多聚甲醛固定液,免疫组化法检测大鼠顶区皮质、下丘脑5-HT1AR、D3R阳性细胞。结果:经前平颗粒能使模型大鼠顶区皮质、下丘脑5-HT1A和D3受体阳性细胞明显增多(P<0.05)。结论:经前平颗粒可通过调节中枢5-HT1AR和D3R受体的表达,治疗PMS肝气逆证及相关的情志障碍。
Objective： To approach the mechanism by observing the effects of Jingqianping granules on the expression levels of hypotypes of 5-hydroxytryptamine receptors （5-HTIAR） and dopamine receptors （D3R） in hypothalamuses and parietal region cortexes of the premenstrual syndrome （PMS） liver-qi invasion model rats. Methods： The rat model of PMS liver-qi invasion was established for the administrations of Jingqianping granules. After the treatment, the heart was perfused with 4% polyoxymethylene, and the positive cells of 5-HT1AR and D3R were checked in the hypothalamuses and parietal region cortexes by immunohistochemistry. Results： Jingqianping granules can increase the masculine cells of 5-HT1AR and D3R in the hypothalamuses and parietal region cortexes of the model rats. Conclusions： Jingqianping granules can cure premenstrual syndrome liver-qi invasion and relative affectional disorders by accommodating the central expressions of-HT1AR and D3R.
出处
《药学与临床研究》
2010年第2期152-155,共4页
Pharmaceutical and Clinical Research
基金
国家自然科学基金(30930110
30973688)
