狂犬病抗血清WHO标准品和国家标准品用于RFFIT方法的比较

The comparison of the WHO standard rabies immunoglobulin and the national standard Human rabies immunoglohulin used in the rapid fluorescent focus inhibition test （RFFIT）

目的比较狂犬病快速荧光灶抑制试验（RFFIT）方法中狂犬病免疫球蛋白WHO标准品和狂犬患者免疫球蛋白国家标准品为参照所得结果的差异。方法在同一次RFFIT试验中同时设置WHO标准品参照和国家标准品参照，同时测定12份待测人血清，比较两种标准品所产生荧光灶的百分比；按照中和抗体滴度计算公式计算不同标准品参照所得12份待测血清抗体滴度，比较其差异。结果结果显示WHO标准品和国家标准品的50％感染量均出现在第5孔和第6孔之间，但国家标准品荧光灶百分比低于WHO标准品；以WHO标准品做参照所得到的同一份血清滴度略高于国家标准品。结论WHO标准品和国家标准品在RFFIT方法中存在差异，但不影响结果判定。

Objective Compare the difference of the results referred to the WHO standard rabies immunoglobulin and the national standard human rabies immunoglobulin used in the rapid fluorescent focus inhibition test （RFFIT）. Methods Setting the WHO standard immunoglobulin and the national standard immunoglobulin in the same system and testing 12 human serum at the same time. Compare the fluorescence percentage of the two different standard immunoglobulin; compare the 12 serum results calculated from the two different standard immunoglobulin used the calculation formula of neutralization antibody titer. Results The Results display that the 50% percent of the two standard immunoglobulin are all between the fifth and the sixth well, but the percentage of the national standard immunoglobulin is lower than the WHO one. The same testserum result calculated from the WHO standard immunoglobulin is little higher than the national one. Conclusion There is difference in the WHO standard immunoglobulin and the national one, but there is no influence in the results.