超声波法提取猴头下脚料多糖工艺研究

Study on Extraction Technology of Intracellular Polysaccharide from Byproduct of Hericium erinaceus by Ultrasonic Wave

[目的]综合利用猴头下脚料和残次菇,筛选出超声波法提取多糖的最佳工艺。[方法]通过粉碎、超声提取、浓缩、Sevag法除蛋白、醇沉纯化等得到多糖,采用硫酸-蒽酮法测定多糖含量。在设计单因素试验优化多糖提取工艺的基础上,采用正交试验确定超声波法提取猴头下脚料多糖的最佳工艺。[结果]pH值在5.0～6.5范围内,经正交试验所得超声波法提取猴头下脚料中多糖的最佳工艺为:料液比(体积比)为1∶70,超声功率为最大功率的80%,提取时间是45min,提取温度为60℃,此时多糖提取率可达9.56%。[结论]该工艺条件综合利用下脚料和残次菇,多糖得率较高,有一定的开发利用价值。

[Objective] To determine the optimum ultrasonic wave extraction technology of polysaccharide from byproduct of Hericium erinaceus. [Method] Polysaccharide was obtained by crushing,ultrasonic extraction,concentration,sevag method,and alcohol extraction,and polysaccharide contents were determined by the anthrone-sulfuric method. Based on the polysaccharide extraction process of optimization of single-factor test,using orthogonal test to determine the optimum extraction technology of intracellular polysaccharide from by product of Hericium erinaceus by ultrasonic wave.[Result] While pH value was from 5 to 6.5,the optimum technology of extracting polysaccharide from byproduct of Hericium erinaceus by ultrasonic method： solid-liquid ratio （volume ratio） was 1∶70; ultrasonic power was 80% of the maximum power;extraction time was 45 min,extraction temperature was 60 ℃,and the extraction rate of polysaccharide from byproduct of Hericium erinaceus was up to 9.56%. [Conclusion] Extraction technology of polysaccharide from byproduct of Hericium erinaceus had a certain degree of development and utilization value,it provided the theoretic basis for the comprehensive utilization of the wastes and byproduct of Hericium erinaceus.