香椿叶总黄酮提取工艺的研究

Study on Extraction Technological Conditions of Total Flavonoids from Toona sinensis Leaves

[目的]研究香椿叶中黄酮类化合物的提取技术,以期为香椿叶在食品和医药领域的进一步开发提供理论依据。[方法]试验以芦丁为标准品,采用分光光度法测定了香椿叶中的总黄酮含量。通过单因素试验分析了乙醇浓度、料液比、浸取温度与浸取时间4个主要因素对香椿叶总黄酮提取率的影响,并在单因素试验的基础上通过正交设计法优化了香椿叶总黄酮提取工艺条件。[结果]香椿叶总黄酮的最佳提取工艺条件为:浓度为70%乙醇作为提取溶剂,料液比1∶50,浸提温度70℃,浸提时间2.0h,此条件下香椿叶总黄酮的提取率可达5.84%。[结论]在最佳提取工艺条件下重复5次试验,总黄酮的平均得率为5.84%,故该方法稳定可靠。

[Objective] The aim was to study extraction technological conditions of total flavonoids from Toona sinensis leaves in order to provide theoretical basis for further development of T. sinensis leaves in the field of food and medicine. [Method] Spectrophotometry was adopted to determine total flavonoids in T. sinensis leaves. By using single-factor test,effects of ethanol concentration,solid-liquid ratio,temperature and time on extraction rate of total flavonoids were analyzed. Based on this,the extraction technological conditions of total flavonoids from T. sinensis leaves were optimized through orthogonal design method. [Result] The results showed that the optimal extracting conditions were as follows：ethanol concentration 70%,solid-liquid ratio 1∶50,extracting duration 2.0 h and refluxing temperature 70 ℃. Under these optimum experimental conditions,total flavonoids yield from T. sinensis leaves could reach 5.84%. [Conclusion] The test was repeated for 5 times under the optimum extraction conditions,and the mean yield of total flavonoids was 5.84%.Therefore,the method is stable and reliable.