贺州大肉姜的组培快繁技术研究

Tissue Culture and Rapid Propagation of Hezhou Ginger

[目的]研究大肉姜组培快繁技术,为贺州大肉姜规模化生产提供技术支持。[方法]以贺州大肉姜的芽作为外植体,研究不同消毒液和激素组合对外植体污染率、芽分化和生根的影响。[结果]外植体用0.1%HgCl2消毒12min效果最好,诱导芽分化的最佳培养基为MS+2.0mg/L6-BA+0.1mg/LNAA,最佳生根培养基为1/2MS+1.0mg/LNAA,生根率可达100%。[结论]该组培快繁技术可用于贺州大肉姜的规模化生产。

[Objective] To study the tissue culture and rapid propagation technology of Hezhou ginger and provide technical support for large-scale production.[Method] Hezhou ginger （Zingiber officinale Rosc.） buds were used as explants.The effects of different disinfectants and hormonal combinations on contamination rate,bud differentiation and rooting were studied.[Result] The disinfection efficiency of explants were better with 0.1% HgCl2 for 12 min.The optimal medium for bud differentiation was MS＋2.0 mg / L 6-BA＋0.1 mg/L NAA.The best medium for rooting was 1/2MS＋1.0 mg/L NAA,and the rooting rate was up to 100%.[Conclusion] The tissue culture and rapid propagation technology can be used for large-scale production of Hezhou ginger.