感染后肠功能紊乱大鼠肠道DCs和细胞因子IL-1β、IL-4的表达

The intestinal dendritic cells and IL-1β,IL-4 cytokine expression of post-infective intestinal dysfunction of rat

目的建立感染后肠功能紊乱大鼠模型,探讨大鼠肠道免疫耐受功能变化。方法将60只雄性Wistar大鼠随机分为对照组和实验组,用福氏志贺痢疾杆菌灌胃制造感染后肠功能紊乱大鼠模型。然后评价两组大鼠回肠末端和远端结肠大体形态和组织学评分,免疫组化检测肠道表面分子CD11c与共刺激分子CD80、CD86表达,检测IL-1β、IL-4表达。结果建立感染后肠功能紊乱大鼠模型后,免疫组化示实验组大鼠远端结肠CD11c、CD80、CD86表达较对照组均明显增高(P<0.05),且回肠末端和远端结肠IL-1β表达均显著增加(P<0.05)。结论感染后肠功能紊乱大鼠肠道局部免疫耐受功能降低,影响肠道促炎因子与抑炎因子表达失衡,使肠道功能发生紊乱。

Objective To explone the change of rat intestinal immune tolerance through establish the rat model of post-infective intestinal dysfunction.Methods The 60 male Wistar rats were randomly divided into control group and experimental group,the post-infective intestinal dysfunction of rat model was induced by gavage with Shigella flexneri.After infection,we evaluated the the macroscopic and histological scores of terminal ileum and distant colon of two groups.Immunohistochemistry was detected the expression of intestinal surface molecules CD11c andconstimulatory molecules CD80,CD86,and detected the expression of interleukin-1β and interleukin-4.Results After the post-infective intestinal dysfunction of rat model established,immunohistochemistry showed that the expression of CD11c、CD80、CD86 on distant colon of experimental group were significantly increased（P〈0.05）;the expression of interleukin-1β on terminal ileum and distant colon of experimental group were significantly increased（P〈0.05）.Conclusion The intestinal dysfunction of rat after infection,the immune tolerance of its part intestinal may reduce,influence the imbalance between anti-inflammatory and proinflammatory cytokine,make the intestinal function disorder.