大庆油田油藏采出水的细菌群落结构

Bacterial community structure in production water from oil reservoirs in Daqing Oilfield

采用ARDRA(扩增性rDNA限制性酶切片段多态性分析)技术对大庆油田聚驱、水驱和过渡带3种油藏采出水中的细菌群落的基因组总DNA的16SrDNA克隆文库进行分析,研究了细菌群落结构.结果表明:随机挑取的596个阳性克隆可分为85个操作分类单元(OTUs),其中聚驱、水驱和过渡带文库分别含有28、41和33个.通过对优势OTUs测序,并与GenBank进行序列比对,发现油藏采出水中的优势菌群为不动杆菌属、弓形杆菌属、厚壁菌门、假单胞菌属和硫磺单胞菌属.聚驱样品中细菌群落组成最简单,优势菌群为不动杆菌属,占库容的85%,假单胞菌属占7%;水驱样品中的优势菌群也是不动杆菌属,占库容的62%,假单胞菌属和硫磺单胞菌属各占20%和6%;过渡带文库的细菌群落的优势菌群为弓形杆菌属,占库容的50%,不动杆菌属和厚壁菌门各占19%和18%.

By the methods of molecular biology,this paper studied the bacterial community structure in the production waters from polymer-flooded,water-flooded,and transition zone＇s oil reservoirs in Daqing Oilfield of Northeast China.The total DNA was extracted from the three production waters,and the 16S rDNA clone libraries of the waters were constructed.About 200 positive clones were randomly selected from each library,and analyzed with amplified ribosomal DNA restriction analysis（ARDRA）.The selected 596 positive clones were clustered into 85 operational taxonomic units（OTUs）,with 28 OTUs in polymer-flooded library,41 in water-flooded library,and 33 in transition zone＇s library.Sequences analysis of the libraries showed that the dominant bacterial groups in the production waters were Acinetobacter,Arcobacter,Firmicutes,Pseudomonas,and Sulfurospirillum.Polymer-flooded sample had the simplest bacterial composition,with the dominant（85%） of Acinetobacter,and part（7%） of Pseudomonas.Water-flooded sample was also dominated by Acinetobacter（62%）,while Pseudomonas and Sulfurospirillum occupied 20% and 6% of the total,respectively.Different from the former two,the transition zone＇s sample was dominated by Arcobacter（50%）,followed by Acinetobacter（19%） and Firmicutes（18%）.