摘要
目的应用实时定量RT—PCR技术检测X射线全身照射诱导小鼠胸腺Egr-1和Egr-4基因的表达变化。方法小鼠接受X射线全身照射后4和24h,提取胸腺mRNA,以GAPDH作为内参基因,利用实时定量RT—PCR检测方法,探讨Egr-1和Egr-4基因的相对表达量与不同照射剂量之间的关系。同时计数骨髓嗜多染红细胞微核率作为辐射生物剂量参照。结果Egr-1和Egr-4基因表达量在0.5~6Gy全身照射后4和24h均与照射剂量之间存在显著的相关性(r=0.974,0.987,0.978,0.999,P〈0.01),其剂量效应曲线可拟合成线性平方方程。各剂量点Egr-1和Egr-4基因表达量与骨髓嗜多染红细胞微核率高度相关(r=0.866、0.947、0.983、0.835,P〈0.05)。进一步分析表明,照射后4hEgr-4基因表达不仅剂量效应关系良好,且增幅最为明显,并与骨髓嗜多染红细胞微核率的剂量效应曲线相平行。结论实时定量RT—PCR技术检测Egr-4基因的早期表达有可能成为建立大批量、快速辐射生物剂量估算方法的候选者。
Objective To investigate the changes of Egr-1 and Egr-4 gene expression in mice thymus after whole-body irradiation (WBI)with X-rays by real-time quantitative reverse transcriptionpolymerase chain reaction( QRT-PCR). Methods The mRNA was isolated from mice thymus 4 and 24 h after WBI with 0-6 Gy of X-ray irradiation. The changes of Egr-1 and Egr-4 gene expression 4 and 24 h post irradiation were examined with QRT-PCR. Parallelled counts of micronucleus rate in bone marrow polychromatic erythrocytes(PCE)was made as a reference radiation biodosimetric control. Results The relative expression of Egr-1 and Egr-4 genes at 4 and 24 h after WBI with 0. 5 to 6 Gy was changed with the dose ( r = 0. 974,0. 987,0. 978,0. 999, P 〈 0.01 ). At all dose points the relative expression of Egr-1 and Egr4 genes was highly correlated with the micronucleus rate of bone marrow PCE ( r = 0. 866,0. 947, 0. 983,0. 835, P 〈 0. 05 ). The dose-effect relationship could be fitted into linear-quadratic model. The expression of Egr-4 gene was significantly increased at 4 h post irradiation and best correlated with PCE micronucleus rate. Conclusions QRT-PCR assay of early expression of Egr-4 gene might be a candidate for fast, high-throughput radiation biodosimetry.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2010年第2期147-151,共5页
Chinese Journal of Radiological Medicine and Protection
基金
卫生部卫生行业科研专项基金(200802018)