双酚A对人早孕绒毛组织结构及β-HCG分泌功能的影响

Study on the Effects of Bisphenol A on the Morphology and β-HCG Secretion of Human Early Villus

[目的]研究双酚A(BPA)水平与人早孕绒毛膜促性腺激素(β-HCG)分泌功能的关系,探讨BPA对绒毛组织可能带来的影响。[方法]以40例早期妊娠(6～8周)的新鲜绒毛组织进行体外培养,培养基中加入不同浓度的BPA:A组为1000×10-6g/L,B组为500×10-6g/L,C组为1×10-6g/L,D组为对照组。取第2、4天的培养液测定β-HCG;第4天将培养的绒毛组织作病理检查。[结果]①随培养时间增加,D组的β-HCG浓度呈递减变化,而其他组呈递增变化,且差异有统计学意义(P=0.001);随BPA浓度的增加,β-HCG的浓度也逐渐增加,各组间第4天与第2天β-HCG的差异有统计学意义(P=0.002)。②D组绒毛结构正常,其他组绒毛合体滋养细胞明显增生,结节数量有不同程度增多,且出现绒毛间质水肿、纤维素样坏死、退行性变化,部分绒毛出现萎缩。[结论]BPA对绒毛组织具有细胞毒性作用,并可影响滋养细胞的分泌功能。

[Objective] To study the effects of bisphenol A（BPA）on the morphology and β-HCG secretion of human early villus and the posible toxicity of BPA to human early villus[. Methods] The human early villus from forty preganancy cases（6-8 weeks）were collected,cultured and treated with BPA at different concentrations. The cultures were divided into 4 groups based on the concentration of BPA. The concentration of BPA was 1 000×10-6 g/L in group A,500×10-6 g/L in group B,1×10-6 g/L in group C respectively,and without BPA in group D as control. The concentration of β-HCG in the supernatant of culture was measured by chemiluminescence immunoassay at day 2 and day 4 of culture,and the cultured villus was stained by HE at day 4.[Results] ① The concentration of β-HCG in the culture was elevated in A,B and C group with increasing culture time,while its concentration in D group was decreased. The concentration of β-HCG was statistically higher in group A,B and C than in group D. The concentration of β-HCG was increased with the increasing BPA.The differences of β-HCG values between day 4 and day 2 among the groups were statistically differen（tP = 0.002）. ② The histologic abnormalities of villus were characterized by increasing syncytiotrophoblast knotting,prominent cytotrophoblast proliferating,basement membrane thickening,fibrinoid necrosis and anaplasia. In addition,atrophy was also observed in some villus.[Conclusion] In culture,BPA is cytotoxic to villus and has effects on the secretion of trophocyte.