果蔬等植物及其果实中β-胡萝卜素的提取与测定

Extraction and determination of β-carotene in plants of fruits or vegetables

建立果蔬等植物及其果实中β-胡萝卜素的HPLC检测方法。采用打浆机及粉碎机,将水果或蔬菜或干果先粉碎或打浆,然后用石油醚提取,用高效液相色谱法测定β-胡萝卜素的质量分数。最低检测限为（2.85×10^-6）g,回收率为95.4%-99.2%,扩展不确定度UPop=0.08。

Determination of β-carotene in plants of fruits or vegetables with HPLC method is established and confirmed.In this research,the fruits or vegetables are smashed to pieces by grinder or beater at first and then extracted with petroleum ether,using high-performance liquid chromatography（HPLC） to obtain the mass fraction of β-carotene.The minimum detectable values in this system is 2.85 × 10^-6 g.The Recovery and expanded uncertainty UPop are 95.4% ~ 99.2% and 0.08,respectively.