蒲公英药材高效液相色谱特征图谱研究

Study on HPLC fingerprint of Herba Taraxaci

目的:建立蒲公英药材高效液相色谱特征图谱。方法:采用HPLC法,流动相为甲醇-0.015%的磷酸水,梯度洗脱,流速1.0mL.min-1,检测波长323nm。结果:以咖啡酸为参照峰,确定9个共有峰,建立了蒲公英药材HPLC特征图谱。结论:所建立HPLC特征图谱具有较好的精密度和重复性,适用于蒲公英药材的质量控制。

OBJECTIVE To establish the fingerprint of Herba Taraxaci by HPLC. METHODS Analysis was performed by RP-HPLC, with a mixture of methyl alcohol and 0. 015% phosphoric acid as mobile phase in gradient mode. Flow rate was 1.0 mL-min^-1, wavelength at 323 nm. RESULTS Nine co-possessing peaks were selected as the fingerprint peaks of Herba Taraxaci with caffeic acid as refference, and the fingerprint of Herba Taraxaci was established by HPLC. CONCLUSION This method with good precision and reproducibility can be applied to the quality control of Herba Taraxaci.