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FGF受体-1和FGF受体-2介导成纤维细胞生长因子-21信号传导 被引量：2

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摘要 成纤维细胞生长因子-21是FGF家族成员,FGF-21与其他FGF家族成员不同,它没有促进细胞分裂的作用,但具有很强的调节血糖作用.本实验旨在系统研究FGF-21的功能受体,以及配体/受体的作用机制,为深入研究FGF-21的生物功能,加快FGF-21从基因到药物的转变奠定理论基础.前期实验表明,FGF-21促进3T3-L1脂肪细胞葡萄糖代谢,对前脂肪细胞无作用,说明3T3-L1脂肪细胞表达FGF-21功能受体.本文以3T3-L1脂肪细胞为靶标,深入研究FGF-21的功能受体.在FGF-21作用下3T3-L1脂肪细胞中的FGF受体1(R1)和FGF受体2(R2)形成特异性异源二聚体.脂肪细胞经FGF-21处理后,R1和R2均迅速发生磷酸化,表明两个受体均被FGF-21激活.siRNA干涉实验表明,抑制R1和R2功能可以抑制FGF-21处理后3T3-L1脂肪细胞Glut1的表达水平,揭示了FGF-21通过R1和R2上调3T3-L1脂肪细胞中Glut1的表达.免疫印迹结果表明,FGF-21可以介导3T3-L1脂肪细胞表面R1和R2的内吞.克隆后测序分析结果表明,3T3-L1脂肪细胞表达的FGF受体为FGFR-1Ⅲc和FGFR-2Ⅲc.结果表明,FGFR-1Ⅲc和FGFR-2Ⅲc是FGF-21的功能受体,参与FGF-21在脂肪细胞中介导的糖代谢活性的信号传导.
作者 任桂萍 尹杰超 王文飞 李璐 李德山
机构地区 东北农业大学生命科学学院
出处 《中国科学：生命科学》 CSCD 北大核心 2010年第4期332-341,共10页 Scientia Sinica(Vitae)
基金 黑龙江省科技厅重点攻关项目(批准号:2006G0461-00)资助
关键词 FGF-21 FGF受体-1 FGF受体-2 葡萄糖代谢 GLUT1
分类号 Q504 [生物学—生物化学]
  • 相关文献

参考文献21

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二级参考文献69

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共引文献22

同被引文献20

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中国科学：生命科学
2010年 第4期

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