摘要
目的诱导骨髓间充质干细胞向软骨细胞分化,初步探索miR130a在骨髓间充质干细胞向软骨细胞分化过程中的调控作用。方法体外TGF-β1诱导骨髓间充质干细胞向软骨细胞分化,免疫荧光法和免疫组化染色鉴定Ⅱ型胶原,阿辛兰染色鉴定氨基葡聚糖。用Real-time PCR法检测细胞miR130a的表达。结果骨髓间充质干细胞在TGF-β1诱导下可分化为软骨细胞。培养7 d后,诱导培养组细胞miR130a表达的水平显著低于培养前的水平(P<0.05)。结论骨髓间充质干细胞体外诱导可以分化为软骨细胞,在向软骨细胞分化的早期,miR130a表达降低伴随着软骨细胞的分化,提示与软骨形成的过程有关。
Objective To evaluate the role of microRNA130a on rat bone mesenchymal stromal cells（BMSCs） during chondrogenic differentiation.Methods BMSCs were induced to differentiate into chondrocytes by transforming growth factor-β1（TGF-β1） in vitro,immunofluorescence and immunohistochemistry were performed to evaluate MSCs differentiation.RT-PCR was performed to analyze microRNA130a expression at different time points.Results microRNA130a was down-modulated during chondrogenesis after BMSCs been cultured with TGF-β1 for 7 days（P〈0.05）.Conclusion During the early stage of BMSC chondrogenic differentiation,mciroRNA130a expression was specifically repressed,suggesting its role in differentiation of rat bone mesenchymal stromal cells.
出处
《基础医学与临床》
CSCD
北大核心
2010年第5期520-523,共4页
Basic and Clinical Medicine
