复方倍他米松增加流感病毒感染小鼠受损嗅黏膜嗅觉标记蛋白表达

Compound Betamethasone enhances the expression of olfactory marker protein in olfactory mucosa of mice injured by influenza virus

目的探讨复方倍他米松对流感病毒感染小鼠受损的嗅黏膜嗅觉标记蛋白(OMP)表达的影响。方法模拟建立流感病毒性嗅觉障碍小鼠模型,分别于第2天和第4天给予复方倍他米松干预,腹腔内注射复方倍他米松,采用免疫组化和Western blot的方法检测嗅黏膜OMP蛋白的表达水平。结果流感病毒感染1组和2组嗅黏膜OMP的表达水平下调,流感病毒感染后复方倍他米松干预1组和2组嗅黏膜OMP的表达水平明显上调。结论复方倍他米松能够增加流感病毒损伤的嗅黏膜OMP的表达。

Objective To investigate the effects of compound Betamethasone on the expression of olfactory marker protein（OMP） in murine olfactory mucosa injured by influenza virus.Methods An animal model was developed by intranasal application of influenza virus to mice.Compound Betamethasone was injected i.p.（3.5 mg/kg） on day 2 and day 4 after the insult.The expression of OMP was tested by immunohistochemistry and Western blot.Results The expression of OMP was significantly downregulated in the olfactory mucosa of influenza virus control group 1 and influenza virus control group 2;the expression of OMP was significantly upregulated in the olfactory mucosa of post-infection compound Betamethasone group 1 and post-infection compound Betamethasone group 2.Conclusion Compound Betamethasone can enhance the expression of OMP in the olfactory mucosa injured by influenza virus.