期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

乙型肝炎病毒核心蛋白作为表位疫苗载体的应用 被引量：4

Hepatitis B virus core protein as an epitope vaccine carrier: a review
原文传递
导出
摘要 乙型肝炎病毒核心蛋白(Hepatitis B viruscore protein,HBc)可以形成二十面体对称的颗粒样结构,由于其N端、C端和主要免疫显性区域(Major immunodominant region,MIR)允许一定程度的缺失和外源插入,并且能够将外源序列重复且高密度地暴露在颗粒的表面,诱发强烈的外源序列特异的体液和细胞免疫反应,从上世纪80年代中期就开始被运用于表位疫苗的研究。以下主要从影响HBc作为表位疫苗载体的因素,包括HBc长度、外源插入位点和表位序列的性质等来介绍HBc作为表位疫苗载体的应用。 Hepatitis B virus core （HBc） proteins have been used as carrier for foreign epitopes since the 1980s. They could self-assemble into icosahedral particles. Foreign epitopes could be inserted into HBc protein in various protein regions, including the N- or C-terminal and the major immunodominant region （MIR）. The factors relevant in the design of HBc particles for vaccine purpose are summarized in this review.
作者 殷瑛 徐俊杰 陈薇
机构地区 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室
出处 《生物工程学报》 CAS CSCD 北大核心 2010年第4期431-438,共8页 Chinese Journal of Biotechnology
基金 国家自然科学基金项目(Nos.30571745 30972772)资助~~
关键词 乙肝核心蛋白 表位疫苗 疫苗载体 基因重组 hepatitis B virus core protein epitope vaccine vaccine carrier gene recombination
分类号 R392 [医药卫生—免疫学]
  • 相关文献

参考文献34

  • 1Pumpens P, Grens E. HBV core particles as a carrier for B cell/T cell epitopes. Intervirology, 2001, 44(2/3): 98-114.
  • 2Pumpens P, Grens E. Hepatitis B core particles as a universal display model: a structure-function basis for development. FEBS Lett, 1999, 442(1): 1-6.
  • 3Riedl P, Stober D, Oehninger C, et al. Priming Th1 immunity to viral core particles is facilitated by trace amounts of RNA bound to its arginine-rich domain. J Immunol, 2002, 168(10): 4951-4959.
  • 4Broos K, Janssens ME, De Goeyse I, et al. Comparison of serum humoral responses induced by oral immunization with the hepatitis B virus core antigen and the cholera toxin B subunit. Clin Vaccine Immunol, 2008, 15(5): 852-858.
  • 5Lee KW, Tan WS. Recombinant hepatitis B virus core particles: association, dissociation and encapsidation of green fluorescent protein. J Virol Methods, 2008, 151(2): 172-180.
  • 6Wynne SA, Crowther RA, Leslie AG. The crystal structure of the human hepatitis B virus eapsid. Mol Cell, 1999, 3(6): 771-780.
  • 7Crowther RA, Kiselev NA, Bottcher B, et al. Three- dimensional structure of hepatitis B virus core particles determined by electron cryomicroscopy. Cell, 1994, 77(6): 943-950.
  • 8Roseman AM, Berriman JA, Wynne SA, et al. A structural model for maturation of the hepatitis B virus core. Proc Natl Acad Sci USA, 2005, 102(44): 15821-15826.
  • 9Zlotnick A, Cheng N, Conway JF, et al. Dimorphism of hepatitis B virus capsids is strongly influenced by the C-terminus of the capsid protein. Biochemistry, 1996, 35(23): 7412-7421.
  • 10Jegerlehner A, Tissot A, Lechner F, et al. A molecular assembly system that renders antigens of choice highly repetitive for induction of protective B cell responses. Vaccine, 2002, 20(25/26): 3104-3112.

二级参考文献23

  • 1Schiller JT, Lowy DR. Papillomavirus-like particle vaccines[J]. J Natl Cancer Inst Monogr, 2000, (28): 50- 54.
  • 2Ulrich R, Nassal M, Meisel H, et al. Core particles of hepatitis B virus as carrier for foreign epitopes [ J ]. Adv Virus Res, 1998, 50:141 - 182.
  • 3Beyer T, Herrmann M, Reiser C, et al. Bacterial carriers and virus-like-particles as antigen delivery devices: role of dendritic cells in antigen presentation[J]. Curr Drug Targets Infect Disord, 2001, 1(3): 287-302.
  • 4Pumpens P, Grens E. Hepatitis B core particles as a universal display model: a structure-function basis for development[J]. FEBS Lett, 1999, 442(1): 1 -6.
  • 5Riedl P, Stober D, Oehninger C, et al. Priming Th1 Immunity to Viral Core Particles Is Facilitated by Trace Amounts of RNA Bound to Its Arginine-Rich Domain[J]. J Immunol,2002, 168(10): 4 951 - 4 959.
  • 6Zlotnick A, Cheng N, Conway JF, et al. Dimorphism of hepatitis B virus capsids is strongly influenced by the C2 terminus of the capsid protein[J]. Biochemistry, 1996, 35(23):7 412-7 421.
  • 7Borisova G, Borschukova Wanst O, Mezule G, et al. Spatial structure and insertion capacity of immunodominant region of hepatitis B core antigen [ J ]. Intervirology, 1996, 39 (1/2):16-22.
  • 8Zlotnick A, Cheng N, Stahl SJ, et al. Localization of the C terminus of the assembly domain of hepatitis B virus capsid protein: implications for morphogenesis and organization of encapsidated RNA [ J]. Proc Nall Acad Sci USA, 1997,94(18) :9 556- 9 561.
  • 9Thomas NW, Jenkins PG, Howard KA, et al. Particle uptake and translocation across epithelial membranes [J ].J Anat,1996,189 ( Pt 3):487-490.
  • 10Schodel F, Peterson D, Hughes J, et al. Hepatitis B virus core particles as a vaccine carrier moiety: Ⅰ. presentation of foreign epitopes[J]. J Biotechnol, 1996;44(1/3) :91 - 96.

共引文献2

同被引文献42

引证文献4

二级引证文献4

生物工程学报
2010年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部