性激素抑制活性氧诱导肝癌细胞增殖的实验研究

Cell proliferation induced by estrogen and androgen inhibiting reactive oxygen species in liver cancer

目的:探讨性激素和活性氧协同作用对肝癌细胞增殖的影响。方法:采用MTT法观察性激素(雌二醇或睾酮)和H2O2单独处理或同时处理表达性激素受体的Hepa1-6细胞的增殖率。结果:浓度<10μmol/L的雌二醇或睾酮对细胞的增殖无显著影响。H2O2浓度为1～15μmol/L时,促进细胞增殖。>100μmol/L则抑制细胞的增殖甚至诱导细胞的凋亡和坏死。雌激素或雄激素与15μmol/LH2O2联合使用时,细胞增殖消失;与200μmol/LH2O2联合使用时,增强了H2O2对细胞的毒性作用。性激素可抑制低浓度H2O2诱导的细胞增殖效应并可加强中等浓度H2O2的细胞损伤作用。结论:性激素与活性氧的相互作用抑制了细胞的增殖,为进一步探讨性激素与活性氧的相互作用关系提供了实验依据,为性激素依赖或相关性肿瘤的治疗提供了新的思路。

OBJECTIVE：To explore the relationship between reactive oxygen species（ROS）and sex hormones on liver cancer cell proliferation.METHODS：The hepa1-6 cells were treated by H2O2 and sex hormones（testosterone and estradiol）separately or simultaneously,and then the cell proliferations were investigated by the MTT method.RESULTS：The sex hormones did not significantly influence the cell proliferation up to the concentration of 10 μmol/L.For H2O2,the cell proliferation significantly improved at the low concentration（1-15 μmol/L）.However,the cell proliferation was significantly inhibited or the cells were induced to death at the high concentration（〉100 μmol/L）.Interestingly,when the cells were treated simultaneously with sex hormones and H2O2,the promotion function of low concentration of H2O2（15 μmol/L）was impaired and the inhibitory function of high concentration of H2O2（20 μmol/L）was promoted by sex hormones.CONCLUSION：Sex hormones impair the promotion function of low concentration of H2O2 to the cell proliferation and promote the inhibition function of middle concentration of H2O2 to the cell proliferation.