鼻咽癌组织中EB病毒定量检测的临床意义

Quantitative detection of epstein-Barr virus infectious status in nasopharyngeal carcinoma tissues

目的:探讨鼻咽癌组织中EB病毒(EBV)定量检测的意义。方法:应用荧光定量PCR(real-timePCR)技术定量检测30例鼻咽低分化鳞癌患者癌组织、癌旁组织及15例正常健康人鼻咽黏膜组织中的EBV拷贝数,并比较三者检测结果。结果:正常人鼻咽黏膜EBV检出率为73.3%(11/15),平均拷贝数为6.5×102μg-1;鼻咽癌和癌旁组织EBV检出率为100%(30/30)和76.7%(23/30),平均拷贝数分别为6.7×105μg-1DNA和1.3×105μg-1DNA。正常人鼻咽黏膜、鼻咽癌患者癌旁组织感染EBV的概率差异无统计学意义(P>0.05);鼻咽癌组织、癌旁和正常黏膜组织3者之间,鼻咽癌与癌旁组织之间,癌旁和正常黏膜组织之间EBV拷贝数差异有统计学意义(P<0.01)。结论:鼻咽癌与癌旁组织感染EBV的数量明显高于正常鼻咽黏膜,EBV感染量是鼻咽癌致病的重要因素,定量检测鼻咽癌组织中EB病毒有助于临床判断病情。

OBJECTIVE：To explore the clinical significance of quantitative detection of Epstein-Barr virus infectious status in nasopharyngeal carcinoma tissues.METHODS：The carcinoma tissues and adjacent tissues from 30 patients with poorly differentiated nasopharyngeal squamous cell carcinoma were prepared simultaneously,and nasopharyngeal tissues from 15 healthy subjects were used as the control.The EBV DNA copies in the samples were tested by using rea1-time quantitative polymerase chain reaction（PCR）.RESULTS：The infectiou rates of EBV were 73.3%（11/15）in nasopharyngeal tissues of healthy subjects（mean EBV DNA copies of 6.5×10^2 μg^-1 DNA）,100%（30/30）in carcinoma tissues and 76.7%（23/30）in adjacent tissues of nasopharyngeal carcinoma patients（mean EBV DNA copies of 6.7×10^5 μg-1 DNA and 1.3×10^5 μg-1 DNA）respectively.There was no significant diference in the infectious rates and levels of EBV between the adjacent carcinoma tissues of nasopharyngeal carcinoma patients and nasopharyngeal tissues of healthy subjects（P〉0.05）.The infectious level of EBV was significantly higher in carcinoma tissues than in adjacent tissues,and significantly higher in adjacent tissues than in normal nasopharyngeal tissues of nasopharyngeal carcinoma patients（P〈0.01）.CONCLUSIONS：EBV latent infection is a common phenomenon in nasopharyngeal tissues of both nasopharyngeal carcinoma patients and healthy persons.The enhancement of EBV infection plays an important role during carcinogenesis of nasopharyngeal carcinoma patients,which sheds further light on the occurrence of EBV with nasopharyngeal carcinoma.