RT-PCR检测OATP-B和OATP-D mRNA在肺癌组织中的表达及其意义

Expressions of organic anion transporting polypeptide B and D mRNA in lung cancers using fluorogenic quantitative RT-PCR and their clinical significance

目的:建立荧光定量RT-PCR法检测肺癌组织中人类有机阴离子转运多肽(OATP)B和DmRNA的表达,并分析其表达与病理分型及临床资料的关系。方法:SYBRGreen实时荧光定量PCR定量检测OATP-B和OATP-D的mRNA在40例肺癌中的表达,分析其在不同病理分型肺癌和正常肺组织间及有无淋巴结转移肺癌间的表达差异,并用10例正常肺组织作对照。结果:与正常肺组织相比,OATP-B和OATP-D的mRNA在肺癌中表达均上调,差异有统计学意义,P<0.05;OATP-B和OATP-D的mRNA在有无淋巴结转移肺癌中的表达差异有统计学意义,P<0.05。结论:肺癌中OATP-B和OATP-D的mRNA表达均明显高于正常肺组织,且OATP-B和OATP-D的mRNA在淋巴结转移肺癌中的表达均高于无淋巴结转移肺癌。

OBJECTIVE： To establish a method for detecting the expressions of OATP-B and OATP-DmRNA in lung cancers via real-time FQ-RT-PCR and to analysis their relerationship with the clinical data in order to evaluate the clinical significance.METHODS： The expressions of OATP-B and OATP-DmRNA in 40 primary lung cancers and 10 normal lung tissues were detected by SYBRGreen quantitative real-time PCR.The expression differences of OATP-B and OATP-DmRNA between lung cancers and normal lung tissues were studied,and the relerationship between the expressions and their clinical data was also analyzed by the statistical method.RESULTS： The expressions of OATP-B and OATP-DmRNA were all signicantly up-regulated in the lung cancers compared with those in the normal lung tissues（P〈0.05）.There was also a significant difference in the expressions of OATP-B and OATP-DmRNA between lymph metastasis lung cancers and those without lymph metastasis（P〈0.05）.CONCLUSION： Compared with normal lung tissues,the expressions of OATP-B and OATP-DmRNA are all significantly up-regulated in lung cancers,and the expression level in lymph metastasis lung cancers is higher than that without lymph metastasis.