聚羟基丁酸酯可吸收缝线生物相容性的实验室研究

Investigation of the biocompatibility of polyhydroxybutyrate absorbable suture in laboratory

目的:研究聚羟基丁酸酯(Polyhydroxybutyrate,PHB)可吸收缝线的生物相容性。方法:①将成纤维细胞进行体外培养,传代后与PHB可吸收缝线复合培养,设与经聚氰基丙烯酸酯处理过的PHB可吸收缝线复合培养为对照组,观察细胞生长情况;②行扫描电镜检查观察细胞在丝线上的生长情况;③以MTT法检测复合培养后的细胞活性。结果:①实验组细胞生长良好,对照组细胞漂浮,收缩成团;②扫描电镜下见细胞在丝线上贴附生长良好;③MTT法检测实验组对细胞增殖无明显影响。结论:PHB可吸收缝线具有良好的生物相容性。

Objective To investigate the biocompatibility of absorbable suture made of Polyhydroxybutyrate（PHB）.Methods The fibroblast were co-cultured with PHB suture verse fibroblast co-cultured with PHB suture which had been dipped in the cyanoacrylate sdhesive（for cytotoxicity）,the proliferation and the adhering condition are observed by inverted microscope and scanning electronic microscope,Culture fibroblast in culture plates containing PHB suture,investigate the attachment rate with MTT assay.Results The number of fibroblast increased co-cultured with PHB,and the comparison cells were floated and shrinked,by scanning electronic microscope,there were cells connect each other adhering on the surface of the suture.Conclusion PHB have fine biocompatibility with fibroblast.