芩丹胶囊对血管外膜成纤维细胞的作用机制

Effect of Qindan Capsule on arterial adventitial fibroblasts

目的探讨芩丹胶囊(QC)含药血清对动脉血管外膜成纤维细胞(AF)的作用机制。方法采用组织贴块法体外原代培养大鼠胸主动脉成纤维细胞,MTT法观察大[750mg/(kg·d)]、小[150mg/(kg·d)]剂量的QC含药血清对转化生长因子β1(TGF-β1)诱导的大鼠胸主动脉成纤维细胞的增殖的影响;实时定量PCR及western-blot分别检测α-平滑肌肌动蛋白(α-SMactin)和Smad3的基因及蛋白表达情况。结果大、小剂量QC含药血清呈剂量依赖性抑制TGF-β1诱导的AF的增殖(P<0.05),且用药组α-SMactin和Smad3基因及蛋白的表达较单纯刺激组显著降低(P<0.05)。结论QC含药血清通过干扰TGF-β1/Smad信号转导通路抑制AF增殖和分化,从而改善和逆转动脉血管外膜重构。

Objective To investigate the effect and mechanism of the Qindan Capsule-containing serum on adventitial fibroblasts（AFs）. Methods AFs were cultured by tissue explant in vitro and induced by TGF-β1. The proliferation of AFs affected by the Qindan Capsule-containing serum with high[750 mg /（kg·d）]or low[150 mg /（kg·d）]dose was detected by MTT. Protein and mRNA expressions of α-SM actin and Smad3 were observed by Western-blot and Real-time PCR respectively. Results Both high and low doses of the Qindan Capsule-containing serum could reduce proliferation of AFs induced by TGF-β1（P〈0. 05） and inhibit expressions of mRNA and protein of α-SM actin and Smad3（P〈0. 05）. Conclusion The Qindan Capsule-containing serum has significant improved and reverse effect on aortic vascular remodeling. The mechanism may be related to inhibiting proliferation and differentiation of AFs through the TGF-β1 /Smad signaling pathway.