塞来昔布对宫颈癌Hela细胞放射增敏效应的研究

Irradiation enhancement of celecoxib on human cell line of cervical cancer

目的:探讨塞来昔布(Celecoxib)对人宫颈癌Hela细胞的放射增敏作用及其机制。方法:用四唑盐比色法(MTT)观察塞来昔布对Hela细胞的生长抑制情况;克隆形成实验检测放射增敏作用,流式细胞仪检测单用塞来昔布、放射以及二者联合使用时Hela细胞凋亡率和细胞周期变化。结果:MTT检测结果提示塞来昔布对宫颈癌Hela细胞的抑制作用呈剂量和放射强度依赖性;克隆形成实验提示塞来昔布对Hela细胞株有一定程度的放射增敏效应,塞来昔布+放射(D+R)组较单纯放射(R)组相比,放射敏感性指标SER、Do、Dq均呈现不同程度的下调;流式细胞分析显示:Hela细胞周期分布及凋亡率在塞来昔布(D)组、单纯放射(R)组及塞来昔布+放射(D+R)组中均有差异,表现为G0/G1期增加,S期减少,凋亡率增加。结论:塞来昔布对人宫颈癌Hela细胞有明显的放射增敏作用,为临床放疗及与塞来昔布联合运用提供了实验依据。

Objective：To investigate the radiation enhancement effect of celecoxib on human cervical carcinoma Hela cells and its possible mechanism. Methods： MTT assay was employed to examine the growth suppression of Hela cells. The enhancement of radiosensitising effect was detected by clone formation experiment. The apoptosis rate and change in cell cycle of Hela ceils treated with celecoxib or/and radiation were examined by flow cytometer. Results： The outcome of MTr assay suggested that celecoxib may produce a concentration - dependent and radiation dose - de- pendent inhibition of Hela cell growth. By colony - forming experiments, celecoxib showed radiosensitizing effect to a certain extent in Hela cells. The survival curve and the related parameters indicated that celecoxib could enhance the radiosensitivity of Hela cells. The value of Do and Dq of Group D ＋ R was significantly different from group R. Flow - cytometric analysis showed cell cycle redistribution and the apeptosis rate had diffrent in group D, R and D ＋ R,they could change cell cycle of Hela cells lines with G0/G1 phase cells increasing and S phase cells decreasing and the ap- optosis rate increasing. Conclusion：Celecoxib possesses radiosensitizing effect on Hela cells in vitro, which presents a laboratory evidence for combined administration of celecoxib and irradiation to treat human cervical carcinoma.