SULT1E1蛋白表达和性激素含量与子宫肌瘤发病的相关性研究

Correlation research on the expression of SULT1E1,contents of sex hormone and pathogenesis of hysteromyoma

目的:探讨性激素及人雌激素硫酸基转移酶在子宫肌瘤发生、发展中的作用。方法:化学发光分析法测定30例子宫肌瘤患者和30例同年龄段健康女性血清及组织匀浆中E2、P、T含量。免疫组化染色法检测SULT1E1蛋白的表达。结果:肌瘤组血清E2含量明显低于健康女性组(P<0.05),其T含量则明显高于健康女性组(P<0.05),P含量各组无显著差异;肌瘤组织匀浆中E2含量显著高于肌瘤包膜外肌层组织(P<0.01),其P、T含量两组间无显著差异;肌瘤组织中SULT1E1蛋白表达与肌瘤包膜外肌层组织比较差异显著(P<0.05),肌瘤组织与肌瘤包膜外肌层组织中SULT1E1蛋白定量表达与相应组织匀浆中E2含量分别呈负相关(r=-0.533,r=-0.498,P<0.01)。结论:子宫肌瘤的发生、发展与子宫肌瘤组织局部SULT1E1酶活性降低有关。

Objective：To explore the relationship of sex hormone and human estrogen sulfotransferase in the oc- currence and development of hysteromyoma. Methods： The expressions of estradiol （ E2 ）, progesterone （P） and tes- tosterone（T） in serum and tissue of 30 patients with hysteromyoma and 30 healthy women in the same age were meas- ured with chemil analysis. The immunohistochemical stain was used to detect the expression of SULT1E1 Results： The serum E2 of study group was significantly lower than that of control group. T was significantly higher （ P 〈 0.05）, P was no significant difference;The hysteromyoma E2 levels were significantly higher than those of normal hysteromyoma tissue（ P 〈 0.01 ）, P and T contents between the two groups had no significant differences . The SULT1 E1 expression in hysteromyoma showed negative to weak positive, but in normal uterine tissue it was weak posi- tive to positive, the difference was significant （ P 〈 0.05 ）. Conclusion： The lower SULT1 E1 in local uterine tissue may play important role in the occurrence and development of hysteromyoma.