摘要
目的检测胃癌和肠癌患者外周血中生存素(survivin)和细胞角蛋白20(CK20)基因的表达,并探讨其与肿瘤诊断、转移之间的关系。方法取胃癌79例,肠癌101例,癌前病变124例(胃56例,肠68例)及正常对照组50例,用实时定量反转录聚合酶链反应(Real-time RT-PCR)方法检测外周血中CK20和生存素基因的转录情况,并分析其与临床病理因素之间的关系。结果正常对照组未检测到CK20和survivin mRNA的转录;良性病变者survivin mRNA的表达率为11.3%,肿瘤组CK20和survivin mRNA表达率分别为胃癌48.1%、87.3%,肠癌48.5%、72.3%,其阳性率与疾病分期及淋巴转移等相关。survivin mRNA高转录者5年生存率显著低于低转录者(P<0.01)。CK20和survivin mRNA表达阳性率高于CEA、CA19-9。结论CK20和survivn mRNA在胃肠癌患者中过表达,可作为诊断微转移和评估预后的客观指标,其诊断外周血微转移优于CEA和CA19-9,联合检测有更高的判断价值。
Objective To detect the expression of CK 20 and survivin gene in peripheral blood of patients with gastric and eoloreetal carcinoma and to discuss the relationship between them and carcinoma. Methods Levels of circulating CEA and survivin mRNA expression from 79 patients with gastric carcinoma, 101 patients with colorectal carcinoma, 124 patients with non- cancerous lesions and 50 normal control were detected by reverse transcription real time polymerase chain reation（RT-PCR） and the date was analyzed clinical data. Results There was no CK 20 and survivin mRNA detected in peripheral blood of normal control,while survivin mRNA expression rate in patients with non-cancerous lesions was 11.3%. In patients with gastric and eolorectal carcinoma, the expression rates of CEA and suvivin mRNA were 48.1% , 87.3% in gastric carcinoma and 48. 5% , 72. 3% in eolorectal carcinoma, respectively. The results were statistically associated with lymph node involvement and staging of the disease. 5 years survival rate was lower in patients with higher survivin mRNA expression. The positive rate and relative coefficients of CEA mRNA and survivin mRNA were higher than the expression of serum CEA and CA19-9. Conclusion CK 20 mRNA and suvivin mRNA were overexpressed in elderly patients with coloreetal carcinoma. They may be also the important markers of metastasis and progonosis. The combination of detecting them may be a more useful tool.
出处
《临床消化病杂志》
2010年第2期67-70,75,共5页
Chinese Journal of Clinical Gastroenterology
