大黄素逆转乳腺癌细胞多药耐药及其对ERCC1蛋白表达的影响

Emodin reversing multidrug resistance in breast cancer cells and its influence on expression of ERCC1

目的:探讨中药大黄素对乳腺癌MCF-7/Adr细胞株多药耐药的逆转作用,及其对细胞中ERCC1蛋白表达的影响,阐明大黄素逆转细胞耐药与ERCC1的关系。方法:采用MTT比色法进行药敏试验,蛋白质印迹法检测ERCC1蛋白的表达。结果:MCF-7/Adr对多柔比星和顺铂的耐药倍数分别为21和11倍,在加入10μg/mL大黄素后,耐药逆转倍数分别为2.86和1.79。10和20μg/mL大黄素处理MCF-7/Adr后第2、4、6、10天,ERCC1蛋白的表达呈逐渐下降趋势,在20μg/mL浓度下降低相对明显。结论:大黄素具有逆转MCF-7/Adr细胞多药耐药的作用,同时还下调了ERCC1的表达水平,并显示了一定的时间依赖性。

OBJECTIVE：To investigate the reversal effect of emodin,a traditional Chinese medicine,on multi-drug resistant breast cancer cell line MCF-7/Adr,and explore its influence on the expression of ERCC1. METHODS：MTT assay was used for drug sensitivity tests,and Western Blot was used for ERCC1 protein. RESULTS：The resistance multiples of adriamycin and cisplatin were 21 and 11 in MCF-7/Adr. The reversal multiples of drug resistance were 2.86 and 1.79,respectively,after emodin（10 μg/mL） added. MCF-7/Adr cells were treated with two concentrations （10 and 20 μg/mL） of emodin,after 2,4,6 and 10 days,the trend of ERCC1 expression was gradually downward,It was more obvious comparatively at the concentration of 20 μg/mL. CONCLUSION：Emodin can reverse the multi-drug resistance on MCF-7/Adr cells,and down-regulate the expression of ERCC1 protein in a time-dependent manner.