淫羊藿甙对缺氧所致成骨细胞凋亡的保护作用

Protective Effect of Icariin on Hypoxia Induced Osteoblast Apoptosis

目的:通过在缺氧条件下体外培养成骨细胞,探讨缺氧对成骨细胞增殖与凋亡的影响以及淫羊藿甙的保护机制。方法:采用酶消化法传代培养乳SD大鼠颅盖骨细胞并建立缺氧模型。实验分2组:组1为单纯缺氧条件下进行的细胞培养,不加入任何药物干预;组2为缺氧条件下加入浓度为10ng/ml的淫羊藿甙与细胞共培养,用台盼蓝染色法分别计数培养5d的细胞数;用流式细胞仪检测2组缺氧5d的细胞凋亡率。结果:缺氧组的细胞数明显少于与淫羊藿甙共培养组,缺氧引起的细胞凋亡率较淫羊藿甙共培养组高。结论:缺氧能抑制成骨细胞的增殖,促进成骨细胞调亡而淫羊藿甙对其具有保护作用。

Objective：To investigate the effects of hypoxia on osteoblasts proliferation and apoptosis and the protective effect of Icariin.Methods：The osteoblasts obtained from the calvaria of neonatal SD rats were cultured by enzyme digestion in a hypoxic environment,group 1 with no treatment and group 2 co-cultured with 10ng/ml Icariin.Trypan blue staining was used to measure the cell number and flow cytometer for the rate of apoptosis in both groups on the 5th day after culture.Results：The cell number of hypoxic group was fewer than that of contrast group,and the rate of apoptosis in hypoxic environment was higher than contrast group.Conclusion：Hypoxia can inhibit the proliferation of osteoblasts and promote the apoptosis of osteoblasts.Icariin can protect osteoblasts from apoptosis.