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大肠癌有丝分裂关卡基因BUB1和BUBR1 mRNA的表达分析 被引量：1

Expression of mitosis checkpoint gene BUB1 and BUBR1 mRNA in colorectal cancer
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摘要 目的研究大肠癌组织中有丝分裂关卡基因BUB1和BUBR1 mRNA的表达。方法采用半定量RT-PCR方法检测36例大肠癌和癌旁正常组织中BUB1和BUBR1mRNA表达水平。结果大肠癌和癌旁正常组织中的BUB1 mRNA拷贝数/β-actin mRNA拷贝数比值分别为0.67±0.33和1.24±0.37;大肠癌和癌旁正常组织中的BUBR1 mRNA拷贝数/β-actin mRNA拷贝数比值分别为0.53±0.25和1.03±0.48。BUB1和BUBR1 mRNA的表达水平在大肠癌中显著低于癌旁正常组织(P<0.05)。结论BUB1和BUBR1基因是大肠癌的肿瘤相关基因,可能在大肠癌发病中具有重要作用。 Objective To study the expression of mitosis checkpoint gene BUB1 and BUBR1 in colorectal cancer.Methods The expression of BUB1 and BUBR1 mRNA in 36 patients with coloerectal cancer and normal colorectal tissues were detected by RT-PCR.Results The ratio of BUB1 mRNA/β-actin in 36 patients with colorectal cancer and normal colorectal tissues were 0.67±0.33 and 1.24±0.37.The ratio of BUBR1 mRNA/β-actin in colorectal cancer and normal colorectal tissues were 0.53±0.25 and 1.03±0.48.The results suggested that the expression of BUB1 and BUBR1 mRNA in colorectal cancer tissues were significantly lower than in normal colorectal tissues（P0.05）.Conclusion BUB1 and BUBR1 gene is related gene of colorectal cancer and may play an important role in colorectal pathogenesis.
作者 陈海军 冯全林 王雪林 吴清 朱震 徐正平 吴江 余晓峰 钱红亮 陆琦
机构地区 南京中医药大学附属昆山中医医院肿瘤外科
出处 《临床肿瘤学杂志》 CAS 2010年第4期330-332,共3页 Chinese Clinical Oncology
基金 2005年昆山市社会发展科技计划项目(KS0519)
关键词 BUB1 BUBR1 半定量RT-PCR 大肠癌 BUB1 BUBR1 RT-PCR Colorectal cancer
分类号 R735.3 [医药卫生—肿瘤]
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参考文献10

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二级参考文献27

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共引文献7

同被引文献17

  • 1李刚强,曹金龙.乳腺癌中Bubl蛋白的表达及临床意义[J].肿瘤,2006,26(8):758-760. 被引量：2
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临床肿瘤学杂志
2010年 第4期

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