nmhaFGF对大鼠肾脏缺血-再灌注ET-1和NO的影响

Effects of non-mitogenic human acidic fibroblast growth factor on ET-1 and NO in renal ischemic-reperfusion rats

目的探讨不同浓度非促分裂人酸性成纤维细胞生长因子(nmhaFGF)对肾脏缺血-再灌注(I-R)损伤后内皮素1(ET-1)和一氧化氮(NO)含量的影响。方法成年SD大鼠35只随机分为假手术(切除左肾,暴露右肾)(SA)组和4个模型组(切除左肾,右肾动静脉夹闭60min)。建模后5min经舌静脉注射nmhaFGF10μg/kg(A组)、20μg/kg(B组)、40μg/kg(C组)或生理盐水1.5ml(D组)。术后24h处死,放射免疫及生化方法检测肾组织和血浆ET-1和NO含量。结果B组皮质和外髓ET-1含量明显低于D组(P<0.05),C组肾皮质、外髓、内髓和血清中ET-1含量都明显低于D组(P<0.01);B、C组肾组织中NO含量明显高于D组(P<0.05)。结论nmhaFGF可以通过降低ET-1和升高NO水平减轻大鼠肾脏I-R损伤。

Objective To study the effects of different concentrations of non-mitogenic human acidic fibroblast growth factor（nmhaFGF） on the contents of ET-1 and NO in renal ischemic-reperfusion（I-R） rats.Methods Thirty-five adult SD rats were divided into sharm operation（SA） group（left kidney resection plus right kidney exposure） and 4 I-R model groups（left kidney resection plus clamping the artery and vein of right kidney for 60 min）.nmhaFGF10 μg/kg（group A）,nmhaFGF20 μg/kg（group B）,nmhaFGF40 μg/kg（group C）,or normal saline 1.5 ml（group D） was injected via lingual vein.The rats were killed 24 h later.The contents of ET-1 and NO in renal tissues and blood were detected with radioimmunoassay and biochemical methods.Results ET-1 in renal cortex and epiopticon was lower in group B than that in group D（P0.05）,which in renal cortex,epiopticon,opticon and serum was lower in group C than that in group D（P0.01）.The content of NO in renal tissue was higher in grops of B and C than that in groupD（P0.05）.ConclusionnmhaFGF could attanuate I-R injury by decreasing ET-1 and increasing NO in renal tissue and blood.