组织工程自体骨修复兔大块骨缺损

Autogenous Tissue Engineering Bone for Segmental Bone Defects in Rabbits

目的:研究组织工程自体骨的植入对大块骨缺损修复的影响。方法:培养兔的自体原代骨髓间充质干细胞,制备壳聚糖-明胶网络/羟基磷灰石多孔材料-重组人BMP2(CS-Gel/HA-rhBMP-2)复合材料。将骨髓间充质干细胞作为种子细胞与CS-Gel/HA-rhBMP-2进行复合。截去兔桡骨中段7mm长度的骨组织,建立骨缺损动物实验模型。实验组(每只兔的右侧)植入CS-Gel/HA-rhBMP-2-种子细胞复合材料,对照组(每只兔的左侧)植入CS-Gel/HA材料,2组均不做内外固定,逐层缝合伤口。分别在术后第4、8和12周对缺损处进行X线评分和组织学评分。结果:术后第4周实验组X线和组织学评分与对照组相比差别无统计学意义,8周和12周实验组X线和组织学评分均优于对照组,差异有统计学意义(P<0.05)。结论:实验中所用移植修复材料具有优异的骨传导性、骨诱导性,可以缩短骨缺损的修复时间。

Objective：To investigate the effect of implantation of autogenous tissue engineering bone on the repairing of segmental bone defects in rabbits. Methods：The rabbit mesenchymal cells were cultured. The autogenous tissue engineering bone with CS-Gel/HA-rhBMP-2 was prepared. The bone mesenchymal stem cells were as seed cells,and were carried out with complex CS-Gel/HA-rhBMP-2. The establishment of animal models of bone defect was to amputate the middle of forelimb 7 mm length of the rabbit bone tissue. In experimental group （the right side of each rabbit）,the implantation was CS-Gel/ HA-rhBMP-2-seed cell in the rabbit segmental bone defect models. In control group （the left side of each rabbit）,the implantation was CS-Gel/HA. No internal and external fixation was done in two groups. The wound was sewn up layer by layer. The X-ray grade and histological score were evaluated after 4,8 and 12 weeks of treatment. Results：There were no significant differences in the X-ray grade and histological score between experiment group and control group after 4 weeks of treatment. However the X-ray grade and histological score were significantly better in experiment group than those in control group at 8 and 12 weeks after treatment （P〈0.05）. Conclusion：The autogenous tissue engineering bone can cure the rabbit segmental bone defects.