摘要
1.以纯化的YadA蛋白〔1、3〕免疫普通小鼠4只,获得小鼠YadA多克隆抗血清;2.利用上述免疫血清建立了YadA多克隆抗体的ELISA检测技术,并对其最适条件进行了观察;3.用纯化的YadA蛋白免疫BALB/C小鼠2只,以免疫BALB/C小鼠的脾细胞与骨髓瘤细胞系SP2/0融合,获得17株分泌YadA-McAb杂交瘤细胞株,将其中4株进行了再克隆,经过半年左右的反复传代及冷冻、复苏测试,抗体分泌稳定;4.Western-bloting分析表明瘤株所分泌的单克隆抗体特异;5.
The purification method of YadA was improved on the basis of primary procedure. (2) 4 white mice were immunized with the purified YadA and the polyclonal antibody to YadA was obtained. (3)ELISA detecting polyclonal antibody to YadA had established and its optimal reacting conditions been observed. (4) 2 BALB/C mice were immunized by purified YadA and the spleen cells of the mice were fused with plasmacytoma SP2/0, Obtained 17 fused cell strains secred YadA- McAb, cloning of 4 strains of them were carried out. Testing results shown the above strains could stably secret the YadA-monoclonal antibody.(5) The result of the Western Blotting analysis proved that the monoclonal antibody secreted by the fused cells was special. (6) The YadA-McAb will be possiblly possessed of the good applied prospects for coagnosis differentiating Yersinia enterocolitica from Brucella.
出处
《地方病通报》
1998年第2期13-15,共3页
Endemic Diseases Bulletin
基金
国家自然科学基金
新疆自然科学基金