组织块法分离山羊表皮干细胞研究

Study on the Isolation of Goat Epidermal Stem Cells by Explant

采用组织块法从山羊耳部皮肤基底层分离表皮干细胞,并通过形态学观察、免疫组化染色以及克隆形成率等方法检测山羊表皮干细胞在DMEM/F12和F12两种不同培养体系中的体外生长特性。结果表明,DMEM/F12是一种适合表皮干细胞体外增殖培养的培养基,在此培养体系中细胞可传代至11代。

The epidermal stem cells were isolated from goat ear skin by epidermal explant and cultured in DMEM/F12 and F12 two different groups . The resultant cells were observed and identified by cell growth curve,immnohistochemical staining and colony-forming efficiency. The results showed that DMEM/F12 was a good cell culture medium for the epidermal stem cells and the epidermal stem cells can survive to the 11 subculture in vitro.