转双价基因毛白杨组培生根过程中内源激素含量对比研究

Comparison Study on the Content of Endogenous Hormones in Transgenic Double Genes of Populus tomentosa Carr. during the Rooting Culture

为探讨转rolB-pttGA20ox双价基因毛白杨生根生理变化,对转rolB-pttGA20ox双价基因毛白杨株系RG-4和对照株系PT-16组培苗生根过程中内源激素含量进行了测定分析。结果表明:RG-4株系在无激素培养基上8 d即可见根突出现,比对照提前3 d,生根率、平均根长及根数均高于对照;其内源IAA含量在不定根形成时增加,根形成后降低,且RG-4在起始和处于峰值时期的内源IAA含量显著高于对照;RG-4的GA3含量在生根前降低,生根后升高,与对照的变化趋势相反;ABA含量在生根过程中波动变化,但起始ABA含量RG-4低于PT-16;RG-4的IAA/ABA在根突形成前保持较高的水平,根突形成后降低。

In order to study the rooting and physiological changes of the transgenic rolB-pttGA20ox double genes Populus tomentosa Carr.,the content of endogenous hormones in the controlled strains PT-16 and the transgenic rolB-pttGA20ox double genes Populus tomentosa Carr.RG-4 were measured.The results showed that in the hormone-free medium,root processus of RG-4 formed in 8 days,3 days earlier than the controlled strains,and the rooting rate,the average length and the number of roots were also higher than those of the controlled strains.The content of endogenous IAA in RG-4 was rising during the formation of adventitious root,but decreasing after the root processus,and the content in the beginning and peak time were significantly higher than those of the controlled strains.The content of GA3 in RG-4 was decreasing before the root formation while increasing after the root formation,which was just opposite to the controlled strains.The content of ABA fluctuated during the root formation,but at the beginning,the content of ABA in RG-4 was lower than that in PT-16.The ratio of IAA and ABA of RG-4 kept a high level before the formation of root processus,but decreased after the root processus.