铜绿微囊藻和蛋白核小球藻对不同形态有机磷的利用及其生长

Utilization of dissolved organic phosphorus and the growth of Microcystis aeruginosa and Chlorella pyrenoidosa

本研究探索了铜绿微囊藻(Microcystis aeruginosa)和蛋白核小球藻(Chlorella pyrenoidosa)在单藻批式培养条件下对三种不同形态的可溶性有机磷化合物:1-磷酸-葡萄糖,环磷酸腺苷(c-AMP)和三磷酸腺苷(ATP)的利用及其生长.结果表明铜绿微囊藻和蛋白核小球藻能够利用这三种有机磷源进行生长.铜绿微囊藻对三种不同形态有机磷源的利用效率由高到低依次是:1-磷酸-葡萄糖,ATP和c-AMP,尤其是对磷酸单酯类磷源——1-磷酸-葡萄糖有极高的利用效率,在该磷源培养条件下微囊藻最大藻浓度能达到正磷酸盐培养条件下的最大藻浓度.小球藻对1-磷酸-葡萄糖和ATP的利用率略高于c-AMP.总体上铜绿微囊藻对这三种有机磷源的利用能力要高于小球藻.碱性磷酸酶与藻利用有机磷的能力有重要的关系,藻对有机磷的利用能力随着胞内酶活的增加而增强.随着富营养化程度的加剧,水体中可溶性有机磷在总磷中的比例也不断升高,铜绿微囊藻对可溶性有机磷的较强利用能力,可能促使其成为富营养化水体中优势种的原因之一.

The growth and the utilization of dissolved organic phosphorus （DOP） of Microcystis aeruginosa and Chlorella pyrenoidosa were studied under unialgal batch culture condition. Three different forms of phosphorus sources ： glucose-l-phosphate, cyclic 3＇5＇-adenosine monophosphate （c-AMP） and adenosine triphosphate （ATP）, were examined in our study. The results imply that although both M. aeruginsoa and C. pyrenoidosa were able to utilize those DOP, M. aeruginosa grew better than C. pyrenoidosa. Particularly, M. aeruginsoa could effectively utilize glucose-l-phosphate, and its maximum cell abundance was as high as that un- der phosphate condition. Compared with c-AMP, glucose-l-phosphate and ATP were more efficiently uptake by algae. It demon- strated that alkaline phosphatase was very important for the utilization of DOP. The proportion of DOP to total dissolved phosphorus was pretty high in the eutrophic lake, therefore, the efficient utilization of dissolved organic phosphors may be a competitive advantage for M. aeruginsosa to become dominant species in this water environment.