甲磺酸伊马替尼对K562细胞bcr/abl、SHIP-2基因表达的影响及意义

The influence and significance of Gleevic on the expression of bcr/abl and SHIP2 gene in K562 cell line

目的研究甲磺酸伊马替尼对K562细胞bcr/abl、SHIP2的表达影响及意义。方法采用定量PCR方法检测甲磺酸伊马替尼干预K562细胞后bcr/abl、SHIP2基因表达变化。采用MTT及Western blot方法分别检测细胞增殖与AKT磷酸化水平的变化。结果随着甲磺酸伊马替尼浓度增加及作用时间的延长bcr/abl基因的表达下降(P<0.01),SHIP2的表达增加(P<0.01)。Akt磷酸化水平降低,细胞增殖能力降低。结论甲磺酸伊马替尼能下调bcr/abl基因的表达;SHIP2表达可能受bcr/abl基因的调节,并可能通过调节PI3K/AKT途径影响细胞的增殖。

Objective To investigate the influence and significance of Gleevic on the expression of bcr/abl and SHIP2 gene in K562 cell line.Methods The quantitative PCR was used to detect the expression of bcr/abl and SHIP2 gene in K562 cells after treated with Gleevic,and MTT and Western Blot were used respectively to detect the proliferation and phosphor-Akt levels of K562 cells after treated with Gleevic.Results With the increase of concentration of Gleevic and the prolangation of action time,the expression of bcr/abl gene was decreased in K562 cells,however,the SHIP2 gene expression was increased.The cell proliferation decreased and the levels of phosphor-Akt was decreased.Conclusion Gleevic can down-regulate the expression of bcr/abl gene.The expression of SHIP2 may be regulated by bcr/abl gene,which may affec cell proliferation by way of PI3K/AKT.