摘要
为探求利用12S rDNA序列进行园蛛分子鉴定的可行性,对园蛛属(Araneus)中的鞑靼园蛛(Araneus tartaricus)、大腹园蛛(Araneus ventricosus)、肥胖园蛛(Araneus pinguis)、花岗园蛛(Araneusmarmoreus)和十字园蛛(Araneus diadematus)的12S rDNA序列片段进行了同源性比较和碱基序列分析,结果表明:5种园蛛在该基因片段的277bp中有49个变异位点较稳定。49个信息鉴别位点可作为5种园蛛种类鉴别的分子标记。
The homology and base sequence of 12S rDNA sequence fragment of Araneus tartaricus, Araneus ventricosus , Araneus pinguis , Araneus marrnoreus and Araneus diadematus were compared and analyzed. The results showed that 49 variable loci at 277 bp of 12 S rDNA sequence fragment were stable, which indicates that 49 information identification loci can be used as molecular markers in identification of 5 Araneus species in Inter Mongolia.
出处
《贵州农业科学》
CAS
北大核心
2010年第4期23-25,共3页
Guizhou Agricultural Sciences
基金
内蒙古师范大学研究生科研创新基金项目"不同保藏方法的蜘蛛标本基因组DNA提取研究"(CXJJS08003)
