摘要
为分离出能降解有机磷农药的有效菌株,从长期施用有机磷农药的土壤中筛选出101株菌株,利用紫外分光光度计在293 nm处测定了分离菌株的降解效率,结合形态学和16 S rDNA序列初步分析了随机挑选的10株分离菌株的遗传多样性。结果表明,长期施用农药的土壤中存在大量能够降解毒死蜱农药的微生物,其中以杆状菌居多,共获得68株杆菌。经初步鉴定,10株菌株分别为葡萄球菌属(Staphylo-coccus)、假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)、芽孢杆菌属(Bacillus)、气单胞菌属(Aero-monas)和黄杆菌属(Flavobacterium)6个属。首次分离出气单胞菌属的毒死蜱降解菌。
101 strains were selected from the soil polluted by organophosphorus pesticides for many years to isolate effective strains to degrade organophosphorus pesticides. The degradation efficiency of the selected strains was determined by the ultra-violet spectrophotometer. The genetic diversity of 10 randomly selected strains was analyzed by determining 16 S rDNA sequence and morphological characteristics. The results showed that there was a lot of microorganism to degrade chlorpyrifos in the soil applied by pesticides, and 68 bacilliform bacteria strains were isolated. 10 randomly selected strains belong to Staphylococcus, Pseudomonas, Enterobacter, Bacillus, Aeromonas and Flavobacterium respectively. The chlorpyrifos degradation strain of Aeromonas is first isolated.
出处
《贵州农业科学》
CAS
北大核心
2010年第4期103-106,109,共5页
Guizhou Agricultural Sciences
基金
贵州省教育厅培育项目"动物及微生物有机磷降解酶基因的分离
鉴定及基因工程研究"[黔教科(2007)001]
贵州省自然科学基金项目"猪对氧磷脂酶调节脂肪代谢的分子机制"[黔科合J字(2008)2129]
关键词
毒死蜱
分离
降解
细菌
贵州
chlorpyrifos
isolation
degradation
bacteria
Guizhou
