抗淋巴细胞血清联合他克莫司诱导大鼠肾脏移植免疫耐受实验研究

Immune tolerance induced by anti-lymphocyte serum combined with tacrolimus in rat kidney transplantation

目的探讨抗淋巴细胞血清(ALS)联合他克莫司(FK506)在诱导大鼠肾脏移植免疫耐受中的作用。方法以SD大鼠作为供体,Wistar大鼠作为受体,建立大鼠肾移植模型。对照组(A组)仅行肾移植,术前术后未予免疫干预;ALS组(B组)术后当天腹腔注射ALS,连续应用至术后第10天;FK506组(C组)术后当天开始应用FK506,连续应用至术后第10天;联合组(D组)联合应用ALS与FK506。术后观测各组受者存活时间、移植肾功能、移植肾血供、免疫耐受状态等。结果D组平均存活时间为(37.0±5.3)d,与A组(7.4±1.6)d、B组(16.3±4.7)d及C组(17.5±5.3)d比较,差异均有统计学意义(P<0.05);手术后1周检查A组移植肾血供差,平均肾血管阻力指数0.80±0.06;B组和C组移植肾血供良好,平均阻力指数分别为0.62±0.07、0.63±0.08;D组移植肾血供良好,术后1周与术后20d平均阻力指数分别为0.61±0.04、0.62±0.03;D组与A组相比较差异有统计学意义(P<0.05)。结论ALS联合FK506能够延长受者存活时间和促进免疫耐受的诱导。

Objective To study the role of anti-lymphocyte serum（ALS） combined with tacrolimus（FK506） in induction of immune tolerance during rat kidney transplantation.Methods A model of rat kidney transplantation was established with SD and Wistar rats as donors and recipients,respectively.Rats in control group（group A） underwent kidney transplantation without immune intervention before and after operation,rats in ALS group（group B） were intra-abdominally injected with ALS on the operation day for 10 days,rats in FK506 group（group C） were given FK506 on the operation day for 10 days,and rats in combined group（group D） received ALS and FK506.The survival time,transplanted kidney function and blood supply,and immune tolerance in different groups of rats were observed after operation.Results The mean survival time of rats in groups D,A,B,and C was（37.0±5.3）,（7.4±1.6）,（16.3±4.7）,and（17.5±5.3）days,respectively（P0.05）.One week after operation,the blood supply was poor in group A with an average renal vascular resistance index of 0.80±0.06,rather good in groups B and C with an average renal vascular resistance index of 0.62±0.07 and 0.63±0.08,respectively,and in group D with an average renal vascular resistance index of 0.61±0.04 and 0.62±0.03 respectively one week and 20 days after operation（P0.05）.Conclusion ALS combined with FK506 can prolong the survival time of receptors and stimulate induction of immune tolerance.