摘要
目的观察痰热清注射液对慢性髓系白血病K562细胞增殖的抑制作用。方法将痰热清注射液倍比稀释成1:2、1:4、1:8、1:16、1:32、1:64、1:128、1:256、1:512共9个浓度组,分别处理增殖期慢性髓系白血病K562细胞,镜下观察不同时间点各浓度组细胞生长情况,应用CCK-8法检测细胞增殖能力,绘制生长曲线,计算抑制率和半数抑制浓度(IC50)。结果痰热清注射液能明显抑制K562细胞增殖,细胞倍增时间为24h,1:2至1:8稀释浓度组的细胞毒性大,细胞基本死亡;抑制作用呈剂量和时间依赖性,IC50约为1:55稀释浓度。结论痰热清注射液对K562细胞具有抑制作用,这为临床应用痰热清注射液治疗血液肿瘤并发感染提供了实验基础。
Objective To investigate the inhibitory effects of Tanreqing(phlegm and heat clearing) injection on proliferation of chronic myelogenous leukemia K562 cell line.Methods Tanreqing injection was diluted to 9 groups at the concentration of 1:2,1:4,1:8,1:16,1:32,1:64,1:128,1:256 and 1:512,respectively.Proliferating chronic myelogenous leukemia K562 cells were treated with Tanreqing injection at different dilution concentrations and their growth was observed at different time points under a microscope.CCK-8 assay was employed to detect the ability of the cells to proliferate and growth curves were plotted for calculating the inhibition ratio and 50% inhibiting concentration(IC50) of Tanreqing.Results Tanreqing injection could significantly inhibit the proliferation of K562 cells.The proliferating time of the cells was 24h.The cytotoxicity of Tanreqing injection was greater in 1:2,1:4 and 1:8 concentration groups with almost all the cells died.Tanreqing injection inhibited the proliferation of K562 cells in a time-and dose-dependent manner and its IC50 was approximately 1:55 dilution concentration.Conclusion Tanreqing injection can inhibit the proliferation of K562 cells,thus laying a foundation for its use in treatment of hematological malignancies complicated by infection.
出处
《军医进修学院学报》
CAS
2010年第5期497-499,共3页
Academic Journal of Pla Postgraduate Medical School
基金
军队"十一.五"基金项目(2007增补)~~