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猪GRIFIN基因克隆与组织分布 被引量：1

Molecular Cloning and Tissue Distribution of Gene GRIFIN in Piglet
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摘要 从仔猪的眼球中克隆GRIFIN基因,研究GRIFIN在仔猪不同组织中的分布情况。结果成功克隆出猪GRIFIN基因,全长466 bp,包含一个完整的开放阅读框架432 bp,编码144个氨基酸。组织分布结果显示GRIFIN在断乳仔猪多种组织均有分布。 The coding sequence of GRIFIN was cloned from the tissue of piglet.The gene expression of GRIFIN in various piglet tissues was amplified by RT-PCR.The result showed that the full length of GRIFIN was 466 bp,including an open reading flame of 432 bp,encoding 144 amino acids.Semi-quantitative RT-PCR analysis showed that GRIFIN mRNA could ubiquitously express in various piglet tissues tested.
作者 孙娅莉 李宏基 韩立强 王伟杰 常磊 郭豫杰 杨国宇
机构地区 河南农业大学牧医工程学院 农业部动物生长发育调控重点开放实验室
出处 《江西农业学报》 CAS 2010年第4期15-17,共3页 Acta Agriculturae Jiangxi
基金 河南省重大科技攻关项目(0522010800)
关键词 仔猪 GRIFIN 基因克隆 组织分布 Piglet GRIFIN Cloning of gene Tissue distribution
分类号 S828 [农业科学—畜牧学]
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参考文献10

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二级参考文献15

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江西农业学报
2010年 第4期

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