电针对局灶性脑缺血模型大鼠脑内STAT3mRNA表达水平的影响

Effects of the Electroacupuncture Therapy on Expression of Stat3mrna After Focal Cerebral Ischemia in Rat Brain

目的:研究STAT3在脑缺血后的信号转导、调控机制以及电针的干预作用。方法:采用热凝闭大鼠大脑中动脉致局灶性脑缺血模型,采用原位杂交的方法检测不同时间段STAT3mRNA表达水平。结果:相较假手术组,造模2h后各指标出现轻微升高,24h后进一步升高,在多个指标上与假手术组相较有显著性差异(P<0.05)。3天后数值下降。电针组的检测结果与模型组的趋势相同,即2h组开始升高,24h组较高,3天组下降。但与模型组相较,各时间段在数值上均高于相应的模型组,具有一定的优势。结论:电针治疗可以提高脑缺血后STAT3mRNA表达水平,激发机体的自我保护机制。同时,还可以维持这样保护机制持续更长的时间。由此可推断:电针对脑功能的改善作用,可能与其上调STAT3mRNA表达,进而减少病灶区神经细胞的凋亡有一定的关系。

Objective： To study STAT3 signal transduction,regulation and control mechanisms after cerebral-ischemia and the role of electro-acupuncture（EA）intervention.Methods： Focal cerebral ischemia （FCI）model was established by middle cerebral artery occlusion（MCAO）with heat—coagulation method.The expression of STAT3mRNA is detected in the different time periods by using in situ hybridization method.Results： Compared to sham-operated group,after 2 hours each indicator of the model one appears slightly increased,and further increased in a number of indicators after 24 hours,there was significant difference compared with the sham-operated group（P〈0.05）.the data decline after 3 days.Electro- acupuncture groups of test results have the same trend with model groups.However,compared with model group,the numerical are higher than the corresponding model group on each time,has certain advantages.Conclusion： EA therapy can increase the expression level of STAT3mRNA after cerebral-ischemia,and stimulate the body＇s self-protection mechanisms.At the same time,EA therapy can also continue to maintain such protection mechanism longer.Thus we can be inferred： the improvement role of brain function by EA therapy may have a certain relationship with increasing of STAT3mRNA-expression,thereby reducing apoptosis of the damaged neuronal.