摘要
目的:采用高效液相色谱法测定丹参醇提取液中丹参素的含量。方法:色谱柱:Agilent C18(250mm×4.6mm,5μm);流动相:甲醇-1%冰醋酸;流速:1mL/min;温度为室温;检测波长:280nm。结果:线性方程为Y=10571X+12.392(r=0.9999),线性范围:10.2-102.0(μg/mL),丹参素的平均含量为58.66μg/mL,RSD=0.73%,回收率100.12%,RSD=0.37%。结论:该法操作简便,精确度高,可靠性强,可作为丹参醇提液中丹参素含量测定的方法。
Objective:To determinate the content of danshensu in compound danshen with HPLC.Method: Column: Agilent C18(250mm×46.5um);mobilc phase methanol-1% acetic acid;flow rate: 1.0mL/ min;temperature: room temperature;detective wavelength: 280nm.Result: Y=10571X+12.392(r=0.9999) linearity range: 10.2~102.0μg/mL.The content of danshensu in compund danshen extract is 58.66μg/mL,RSD=0.73% in this group averge,recovery was 100.12%,RSD= 0.37%.Conclusion:This method could be used as the analysis method for danshensu in danshen alcohol extract.
出处
《辽宁中医药大学学报》
CAS
2010年第5期63-64,共2页
Journal of Liaoning University of Traditional Chinese Medicine
基金
广东省科技计划项目(2005B10401037)