高效液相色谱法测定瘿消颗粒中芍药苷含量

Determination of Paeoniflorin in Yingxiao Granule by HPLC

目的：建立瘿消颗粒中芍药苷的含量测定方法。方法：采用十八烷基硅烷键合硅胶柱,以乙腈-磷酸三乙胺水溶液（15：85）为流动相,检测波长230nm,流速lmL·min^-1。结果：药苷在0.083-0.75μg之间与相应的峰面积呈良好的线性关系（r=0.9999）,平均回收率在95.1%-99.0%之间,RSD为1.5%（n=5）。结论：高效液相色谱法操作简便、准确、重复性好,可用于瘿消颗粒的质量控制。

Objective：To establish a HPLC method for the determination of dracorhodin perchlorate in Yingxiao Granule.Method：ODS column（150mm×6.6mm） was used.The mobile phase was acetonitrile- triethylamine phosphoric acid solution （15：85）,the flow rate was l.0mL/min,the detection wavelength was 230nm.Result： Average recovery is 95.1%~99.0%,RSD is 1.5%（n=5）.Conclusion：The method is simple and accurate with good reproducibility in HPLC.It can be used for quality control of Yingxiao Granule.