苦皮藤素V在植物体内穿透与输导的初步研究

Primary studies on the penetration and translocation of celangulin V in the plants

【目的】了解植物源杀虫活性物质苦皮藤素V在植物体内的穿透与输导作用。【方法】以蚕豆苗、小麦苗为供试植物材料,用苦皮藤素V处理植物根系及叶片,定期取样,采用高效液相色谱(HPLC)检测植物中的苦皮藤素V含量。【结果】苦皮藤素V可以穿透蚕豆苗根系进入植物体内,处理12 h后蚕豆苗根系中苦皮藤素V含量为0.20μg/g,48 h后达到1.72μg/g;苦皮藤素也可以穿透叶片进入植物体内,处理12 h后,蚕豆上层叶片苦皮藤素含量为0.34μg/g,48 h后达到0.77μg/g。水体中的苦皮藤素V可以穿透小麦苗根系进入植物体内,处理8 h后,植物中苦皮藤素V含量为0.29μg/g,36 h后达到2.94μg/g,但由于土壤对苦皮藤素V的吸附作用,小麦根系难以吸收到施于土壤的苦皮藤素V。【结论】苦皮藤素V可以穿透植物根系或叶片在植物体内输导。

【Objective】 The objective of this research was to comprehend the penetration and translocation of botanical insecticide celangulin V in the plants.【Method】 The samples of broadbean or wheat seedling that treated by root system or leaf spraying were collected at regular intervals,and the content of celangulin V in samples was analyzed by high performance liquid chromatography.【Result】 The results showed that celangulin V could be penetrated and translocated in the broadbean seedlings from the roots or leaves.The content of celangulin V in the samples was 0.20 μg/g 12 h after treatment,1.72 μg/g 48 h after treatment by root absorption,and 0.34 μg/g 12 h after treatment,0.77 μg/g 48 h after treatment by leaf absorption.The results also showed that celangulin V in water could be penetrated and translocated in the wheat seedlings from the roots.The content of celangulin V in the samples was 0.29 μg/g 8 h after treatment,2.94 μg/g 36 h after treatment.No celangulin V was detected in the wheat seedling which was grown in soil treated with celangulin V.【Conclusion】 Celangulin V could be penetrated and translocated in the plant from roots or leaves.