营养物质对莱氏野村菌MZ060806生长的影响

Effects of different nutrients on growth of Nomuraea rileyi MZ060806

【目的】了解不同营养物质对莱氏野村菌生长的影响,为其开发利用奠定基础。【方法】以查氏培养基为基础培养基,研究不同碳源(葡萄糖、蔗糖、乳糖、麦芽糖、果糖、木糖、甘露醇、可溶性淀粉)、氮源(酵母浸粉、尿素、牛肉蛋白胨、硝酸钠、硝酸钾、硫酸铵、水解酪蛋白、胰蛋白胨、硝酸铵)、维生素(抗坏血酸、硫胺素、烟酸、吡哆醇、核黄素、生物素、泛酸)及不同碳氮比(5∶1,10∶1,15∶1,30∶1,60∶1,120∶1,240∶1,480∶1)对莱氏野村菌MZ060806菌株菌落直径、菌丝干质量和产孢量的影响。【结果】适宜莱氏野村菌MZ060806菌落生长的最佳碳源是蔗糖,氮源是水解酪蛋白,碳氮比是15∶1,维生素是生物素;有利于菌丝生长的最佳碳源是可溶性淀粉,氮源是酵母浸粉,碳氮比是15∶1,维生素是泛酸、核黄素和抗坏血酸;明显促进产孢量增加的最佳碳源是葡萄糖,氮源是水解酪蛋白,碳氮比是15∶1,维生素是硫胺素。【结论】明确了促进莱氏野村菌MZ060806菌落扩展、菌丝生长和产孢的最佳碳源、氮源、维生素种类及碳氮比。

【Objective】 The Nomuraea rileyi was studied to provide basic data to exploit it in large scale.【Method】 The effects on colony diameter,mycelium dry weight and spore yield of Nomuraea rileyi MZ060806 were studied under different nutritional mediums,which were added with different carbon sources（glucose,sucrose,lactose,maltose,fructose,xylose,mannitol,soluble starch）,nitrogen sources（yeast powder,urea,peptone beef,sodium nitrate,potassium nitrate,ammonia sulfate,casein hydrolysate,tryptone,ammonium nitrate） and vitamins（VC,VB1,VB5,VB6,VB2,VH,VB5）,ratio of carbon-to-nitrogen（5∶1,10∶1,15∶1,30∶1,60∶1,120∶1,240∶1,480∶1）.【Result】 For colony diameter,the best carbon source was sucrose,the best nitrogen source casein hydrolysate,the optimal carbon-to-nitrogen ratio 15∶1,and the best vitamin VH;the best carbon source for mycelium growth soluble starch,the best nitrogen source yeast powder,the optimal carbon-to-nitrogen ratio 15∶1,and the best vitamin VB5;the best carbon source for sporulation quantity was glucose,the best nitrogen source casein hydrolysate,the optimal carbon-to-nitrogen ratio 15∶1,and the best vitamin VB1.【Conclusion】 The optimal kinds of carbon sources,nitrogen sources,vitamins and ratio of carbon-to-nitrogen to accelerate growth were ascertained.