乳化异氟醚对人体外循环血清培养人血管内皮细胞ICAM-1表达变化的影响

The effect of Emulsified isoflurane on the changes of ICAM-l expression induced by CPB serum cultured endothelial cells

目的探讨人体外循环(CPB)血清诱致培养人血管内皮细胞ICAM-1表达及乳化异氟醚对其的影响。方法采用CPB血清致伤培养血管内皮细胞模型,以免疫组化方法观察培养血管内皮细胞ICAM-1表达量。结果各组血管内皮细胞ICAM-1的表达随着时间的延长均呈进行性升高。各时段小牛血清(FBS)组血管内皮细胞ICAM-1表达量最低(P<0.01)。与CPB前血清组相比,CPB后血清组及乳化异氟醚组1h、2h、4h、8h、12h时的ICAM-1表达水平显著升高(P<0.01)。乳化异氟醚组在1h、2h、4h、8h升高的幅度与CPB后血清组相比明显减少(P<0.01),在12h时的ICAM-1表达水平与CPB后血清组相比无显著性差异(P>0.05)。结论CPB显著增加ICAM-1的表达,乳化异氟醚在一定时间内可显著减轻CPB对内皮细胞的影响,减少ICAM-1的表达,对于体外循环后全身炎症反应可能有保护作用。

Objective To observe the effect of Emulsified isoflurane on the changes of ICAM-1 expression induced by CPB serum cultured endothelial cells. Methods ICAM-1 expression on cultured endothelial cells was detected by immunohistochemical method. Results ICAM-1 expression on cultured endothelial cells was increased in every group. ICAM-1 expression in group FBS was the lowest in four groups （P 〈 0.01 ）. ICAM-1 expression in group post-CPB serum and group Emulsified isoflurane was higher than group pre- CPB（P 〈0.01 ）. ICAM-1 expression in group Emulsified isoflurane was lower than group post- CPB in 1 h,2 h,4 h and 8 h. ICAM-1 expression was almost the same between group Emulsified isoflurane and group post-CPB in 12h （P 〉0. 05）. Conclusion Emulsified isoflurane could inhibit ICAM-1 expression on cultured endothelial cells and might play a role in decreasing systemic inflammatory response syndrome induced by CPB.