柴胡注射液抑制呼吸道合胞病毒的研究

The study inhibiting Respiratory syncytial virus administered by Injectio Bupleurum

目的:研究柴胡注射液在细胞培养中抑制呼吸道合胞病毒(RSV)的作用.方法:用细胞培养法,采取Vero细胞感染病毒2小时后给药,在显微镜下观察细胞病变情况.结果:柴胡注射液组与病毒对照组相比,病变程度有显著差异,柴胡注射液抑制RSV的最大无毒浓度(TDo),半数有效浓度(IC_(50)),最小有效浓度(MTC)和治疗指数(TI)分别为1000μg/ml,500μg/ml,250μg/ml和4.结论:柴胡注射液具有明显抑制RSV的作用.

AIM: To study the effect of which Injectio Bupleurum inhibits RSV in the condition of cell culture.Methods: By the method of cell culture and the way of drug administered after two hours when Vero cells were infected by virus, CPE was observed under the microscope. Results: There was significant different CPE when the group of Injectio Bupleurum compared with the control of virus. TD, IC50, MIC and TI of which Injection Bupleurum inhibited RSV was 1000μg/ml, 500μg/ ml,250μg/ml, and4, respectively. CONCLUSIONS: Injection Bupleurum could inhibit RSV significantly.