摘要
目的:探讨半夏提取物对小菜蛾成虫的产卵忌避作用和杀卵作用。方法:采用3种极性不同的溶剂(石油醚、乙酸乙酯和无水乙醇)利用索式提取法从半夏干粉中制备提取物,测定提取物对小菜蛾成虫产卵及卵孵化的影响。结果:半夏乙醇提取物对小菜蛾有很强的产卵忌避作用和杀卵作用,浓度越高,产卵忌避作用越强,卵的存活率越低。在非选择性和选择性试验处理后24h,质量浓度为100g·L-1乙醇提取物对小菜蛾的产卵忌避率分别为85.13%,73.38%;100g·L-1乙醇提取物处理卵后5d,孵化率为32.75%,卵孵化抑制率可达62.40%。结论:半夏提取物对小菜蛾成虫产卵及卵孵化均具有作用,其中乙醇提取物的生物活性显著优于乙酸乙酯和石油醚提取物。
Objective： To study the bioactivities of the extracts from Pinellia ternata against Plutella xylostella. Method： The active material in P. ternata was Soxhlet extracted with petroleum ether, ethyl acetate, ethanol successively. The bioactivities of the extracts were determined. Result： Results indicated that all extracts using three solvents showed inhibition of oviposition and ovicidal action activities, but the activity of ethanol extract was the strongest. The higher concentration, the more obviously the effect on the pest was. 24 h after treatment with 100 g · L^-1 ethanol extract in no-choice tests and choice tests, the deterrent rates were 85.13%, 73.38%, respectively. Furthermore, treatment with 100 g · L^-1 ethanol extract after 5 d could effectively reduced the percentage of eggs incubation, ovicidal rate could reach 62.40%. Conclusion： These results suggest that extracts of P. ternata could be used to control pest.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第9期1108-1111,共4页
China Journal of Chinese Materia Medica
基金
安徽省教育厅自然科学基金项目(kj2007b172
2006jql142)
淮北市科技局项目(05027
070141)
关键词
半夏
小菜蛾
产卵忌避
杀卵作用
Pinellia ternata
Plutella xylostella
iubibting oviposition
ovicidal action
