刮筋板乙酸乙酯部位化学成分

Chemical constituents from Excoecaria acerifclia and their bioactivites

目的:研究刮筋板Excoecaria acerifclia的化学成分和生物活性。方法:采用色谱技术分离纯化,根据化学成分理化性质和波谱数据确定化学结构;MTT法测定化合物对人肝癌细胞系(human hepatocarcinoma cell line)HepG2的体外抑制作用,用半数抑制浓度(IC50)评价其抗肿瘤活性。结果:分离鉴定了8个化合物,分别为6-甲氧基-7-羟基香豆素(1),6,7-二甲氧基-8-羟基香豆素(2),山柰酚(3),山柰酚3-O-吡喃葡萄糖苷(4),槲皮素-3-O-β-D-葡萄糖糖苷(5),山奈酚-3-O-β-D-葡萄糖糖苷-2″-没食子酸酯(6),β-谷甾醇(7),胡萝卜苷(8)。生物活性研究显示化合物5和6对人肝癌细胞系HepG2的IC50分别为7.13,65.42μmol·L-1。结论:所有化合物均为首次从该植物中分得。化合物5具有较好的体外抑制HepG2作用。

Objective： To investigate the chemical constituents of Excoecaria acerifclia and their antitumor activities. Method： The constituents were isolated and purified by column chromatography. Their structures were identified by their physicochemical proper- ties and spectral features. Cytotoxicities of the purified compounds were evaluated by MTT method against human cancer cell lines HepG2. Result： Eight compounds were isolated and identified as： 7-hydroxy-6-methoxy- coumarin （1）, 8-hydroxy-6,7-methoxy-coumarin （2）, kaempferol （3）, kaempferol-3-O-β-D-glucoside （4）, quercetin-3-O-β-O galactoside （5）, kaempferol-3-O-β-D-glucoside-2″-gallate （6）, β-sitosterol （7）, daucosterol （8）. Conclusion： All compounds were isolated from this plant for the first time. Compounds 5 showed inhibitory activity towards HepG2 with IC50 values of 7. 13 mol · L^-1.